Co-inherited novel SNPs of the LIPE gene associated with increased carcass dressing and decreased fat-tail weight in Awa
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Co-inherited novel SNPs of the LIPE gene associated with increased carcass dressing and decreased fat-tail weight in Awassi breed Tahreer M. Al-Thuwaini 1
&
Mohammed Baqur S. Al-Shuhaib 1
&
Frederic Lepretre 2 & Zainab A. Mahdi 1
Received: 6 January 2020 / Accepted: 11 September 2020 / Published online: 17 September 2020 # Springer Nature B.V. 2020
Abstract The lipase E hormone-sensitive (LIPE) enzyme is one of the lipolytic enzymes, and it plays a key role in the regulation of adipose tissue deposition. This study was conducted to investigate the possible association between the LIPE gene variations and the main body weight measurements in Awassi sheep. A total of 160 of sexually mature Awassi rams (Ovis aries) that aged between 2 and 3 years were included in the present study. Genomic DNA was extracted and two specific PCR amplicons were designed to amplify two coding regions within the LIPE gene. Genotyping experiments were performed using polymerase chain reactionsingle-strand conformational polymorphism (PCR-SSCP). Two different SSCP banding patterns were identified, CC and CD in exon 2, and AA and AT in exon 9. Five novel single-nucleotide polymorphisms (SNPs) were detected by sequencing, namely g.151C > A and g.198C > T in exon 2, and g.213G > C, g.226G > T, and g.232A > C in exon 9. Haplotype block analysis showed strong linkage disequilibrium values between the two SNPs in exon 2 and the three SNPs in exon 9. Association analysis of haplotypes with carcass traits demonstrated a significantly higher dressing percentage (P < 0.05) and lower fat tail weight (FTW) in CACT and GCGTAC haplotypes made these haplotypes more favorable for human consumption. The current research is the first one to report a tight association between the LIPE genetic polymorphism and the dressing percentage and FTW traits, suggesting a pivotal role played by these co-inherited SNPs in the metabolism of carcass traits in sheep. Keywords Awassi . Carcass dressing . HSL gene . Haplotype
Introduction Lipolysis in adipose tissue plays a major role in energy homeostasis and body weight and is regulated, at least in part, by the multiple lipolytic genes (Nishizawa and Shimomura 2019;
* Tahreer M. Al-Thuwaini [email protected]; [email protected] Mohammed Baqur S. Al-Shuhaib [email protected]; [email protected] Frederic Lepretre [email protected] Zainab A. Mahdi [email protected] 1
Department of Animal Production, College of Agriculture, Al-Qasim Green University, Al-Qasim, Babil 51001, Iraq
2
Plateau de genomique fonctionnelle et structurale, CHU Lille, University of Lille, Lille, France
Kulyte et al. 2020). Lipolytic genes are shown to be related to several body weight measurements in a variety of animal species, and thus, they have the potential to provide an insight into growth traits in sheep (Carlsson et al. 2006). Lipolytic genes have recently demanded more attention because of their inevitable role in obesity and diseases (Luglio et al. 2015).
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