Co-regulation Role of Endogenous Hormones and Transcriptomics Profiling Under Cold Stress in Tetrastigma hemsleyanum
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Co‑regulation Role of Endogenous Hormones and Transcriptomics Profiling Under Cold Stress in Tetrastigma hemsleyanum Xin Peng1,2 · Mingjie Li2 · Hao Wu1,3 · Hongjiang Chen1,3 · Zhongyi Zhang2 Received: 15 July 2020 / Accepted: 12 October 2020 © Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract Tetrastigma hemsleyanum Diels et Gilg is a valuable medicinal herb. Chilling sensitivity is the major limiting factor of the artificial cultivation of the plant. Some key genes, such as ZEP and NCED genes of ABA biosynthesis, GA2ox, GA3ox, and GA20ox genes of GA biosynthesis, ACO genes of ET biosynthesis pathway were screened to be crucial in cold response. The response of ABA and ABA/GA1+3 to cold stress was prior to that of GA1+3, ZR, ABA/IAA, and ABA/ZR. The increasing changes in ABA/GA1+3 turned to a steep decline with the extension of stress time, which might be one factor contributing to cold sensitivity of T. hemsleyanum. The cold tolerance of T. hemsleyanum would be repressed by G A3 but enhanced by ABA when ABA orGA3 was used alone. Both the ABA-mediated promotion and GA-mediated repression of cold tolerance could be attenuated using a combination of the two phytohormones within 6 h. When the biosynthesis of ABA and GA were inhibited by FLU and PAC, respectively, the effects of GA and ABA treatment were reversed partially. In summary, we presented the first study of global hormone-regulated transcripts expression patterns in T. hemsleyanum. This study suggested that GA and ABA could work antagonistically to balance the cold stress responses. PAC, a GA biogenesis inhibitor, as well as exogenous ABA, might be potential plant growth regulators that can promote cold tolerance of T. hemsleyanum. The study also provided valuable hints in revealing the potential candidate genes that regulated cold tolerance of T. hemsleyanum. Keywords Tetrastigma hemsleyanum · Transcriptome · Cold stress · Phytohormone
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00344-020-10246-6) contains supplementary material, which is available to authorized users. * Xin Peng [email protected] Mingjie Li [email protected] Hao Wu [email protected] Hongjiang Chen [email protected] Zhongyi Zhang [email protected] 1
Ningbo Institute of Zhejiang University, Ningbo 315100, Zhejiang, China
2
Fujian Agriculture and Forestry University, Fuzhou 350000, Fujian, China
3
Zhejiang Pharmaceutical College, Ningbo 315100, Zhejiang, China
Abbreviations ABA Abscisic acid GA Gibberellic acid CK Cytokinin ZR Zeatin riboside PAC Paclobutrazol ET Ethylene JA Jasmonic acid BR Brassinosteroid FLU Fluridone AAO3 Abscisic-aldehyde oxidase CESA Cellulose synthase A ZEP Zeaxanthin epoxidase EUPL E3 ubiquitin-protein ligase ELP ETO1-like protein LOX2S Lipoxygenase AOC Allene oxide cyclase 4CL 4-Coumarate–CoA ligase EKES Ent-kaur-16-ene synthase ATH1 Homeobox protein ATH1 ACDD Acyl-CoA dehydrogenase KCT 3-Ketoacyl-CoA thiolase
13
Vol.:(0123456789)
NCED 9-cis-Epoxy
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