Transcriptome Profiling of Gold Queen Hami Melons under Cold Stress
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Transcriptome Profiling of Gold Queen Hami Melons under Cold Stress Q. Zhanga, C. H. Shana, M. Ninga, X. X. Zhaoa, H. F. Dua, W. C. Caia, and F. X. Tanga,* a
Food College, Shihezi University, Xinjiang, 832003 China *e-mail: [email protected]
Received September 30, 2019; revised March 7, 2020; accepted March 16, 2020
Abstract—Hami melon (Cucumis melo L. var. inodorus Jacq) is an important horticultural crop but susceptible to chilling injury during low temperature storage. To characterize the gene expression profiles of Gold Queen Hami melon under the cold stress and discover the key cold stress-induced genes, five cDNA libraries, C0 (control), C12 (control for 12 d), C24 (control for 24 d), L12 (cold treatment at 0.5°C for 12 d) and L24 (cold treatment at 0.5°C for 24 d), were constructed for RNA sequencing (RNA-Seq) and gene expression profiling. A total of 6479 and 7914 differentially expressed genes (DEGs) in L12 and L24 compared with C0 were respectively detected. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses identified the relevant biological functions and metabolic pathways of these differentially expressed genes. In addition, a diverse range of cold response candidate genes were mainly obtained from starch and sucrose metabolism, pentose phosphate pathway and glycolysis/gluconeogenesis, such as genes encoding sucrose-phosphate synthase, α-amylase, β-amylase, sucrose synthase and glucose-6-phosphate dehydrogenase. Various transcription factors with divergent expression patterns were identified. Finally, quantitative real time polymerase chain reaction (qRT-PCR) was conducted to validate the RNA-Seq and gene differentially expression results. This study provides valuable information for further studies on cold response mechanisms and genetic improvement of cold tolerance in Gold Queen Hami melons. Keywords: Cucumis melo, transcriptome, cold response, differentially expressed gene, transcription factors DOI: 10.1134/S1021443720050209
INTRODUCTION Hami melons (Cucumis melo L. var. inodorus Jacq) are unique fruit in Xinjiang, China, and are also known as the “King of the melons”. Low-temperature storage is an effective method that can maintain postharvest quality and prolong post-harvest lifespan in fruits and vegetables [1]. However, Hami melons are sensitive to chilling [2]. Cold stress is a common environmental stress that affects plant growth and postharvest lifespan. To handle this type of environmental stress, plants have evolved a series of defense mechanisms. The accumulation of polyamines has been shown to prevent low-temperature injury, as low temperature generally induce changes in the molecular arrangement of membrane lipid [3]. Sugars also seem to play a potential role in preventing chilling injury. Abbreviations: C0—control; C12—control for 12 d; C24—control for 24 d; DEGs—differentially expressed genes; FDR—false discovery rate; GO—Gene ontology; KEGG—Kyoto encyclopedia of genes and genomes; L12—cold treatment at 0.5°C for 12 d; L24—cold tr
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