Psychrophilic Pseudomonas helmanticensis proteome under simulated cold stress
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ORIGINAL PAPER
Psychrophilic Pseudomonas helmanticensis proteome under simulated cold stress Saurabh Kumar 1 & Deep Chandra Suyal 2 & Amit Yadav 3 & Yogesh Shouche 3 & Reeta Goel 1 Received: 27 April 2020 / Revised: 9 July 2020 / Accepted: 13 July 2020 # Cell Stress Society International 2020
Abstract Himalayan mountains are distinctly characterized for their unique climatic and topographic variations; therefore, unraveling the cold-adaptive mechanisms and processes of native life forms is always being a matter of concern for scientific community. In this perspective, the proteomic response of psychrophilic diazotroph Pseudomonas helmanticensis was studied towards lowtemperature conditions. LC-MS-based analysis revealed that most of the differentially expressed proteins providing cold stress resistance were molecular chaperons and cold shock proteins. Enzymes involved in proline, polyamines, unsaturated fatty acid biosynthesis, ROS-neutralizing pathways, and arginine degradation were upregulated. However, proteins involved in the oxidative pathways of energy generation were severalfold downregulated. Besides these, the upregulation of uncharacterized proteins at low temperature suggests the expression of novel proteins by P. helmanticensis for cold adaptation. Protein interaction network of P. helmanticensis under cold revealed that Tif, Tig, DnaK, and Adk were crucial proteins involved in cold adaptation. Conclusively, this study documents the proteome and protein-protein interaction network of the Himalayan psychrophilic P. helmanticensis under cold stress. Keywords Cold stress . Psychrophile . Bacterial cold adaptation . LC-MS . Himalaya
Introduction Cold ecosystems dominate the Earth biosphere (Kuhn 2012), as 80% of Earth’s environments are permanently under cold stress (temperature below 5 °C), particularly in deep oceans, glaciers, polar regions, and alpines (Feller 2017). Highaltitude terrestrial ecosystems of Western Indian Himalaya (WIH) are one of such cold stressed ecosystems which undergo frequent freezing-thawing cycles (Awasti et al. 2019). Microorganisms in these Himalayan regions are mostly psychrophilic or psychrotrophic in nature and play critical role in
* Reeta Goel [email protected] 1
Department of Microbiology, College of Basic Sciences and Humanities, Govind Ballabh Pant University of Agriculture and Technology, Pantnagar, Uttarakhand, India
2
Department of Microbiology, Akal College of Basic Sciences, Eternal University, Baru Sahib, Sirmaur, Himachal Pradesh, India
3
National Centre for Microbial Resource, National Centre for Cell Science, First floor, Central tower, Sai Trinity building, Pashan, Pune, Maharashtra, India
biogeochemical cycle operating under low temperature (Joshi et al. 2017). Low temperature affects cellular machinery by decreasing membrane permeability, impairing protein folding, and hampering the process of transcription and translation by stabilizing secondary structure of DNA and RNA (Barria et al. 2013; Zhang et al. 2015). Cold-adapted microorganisms have r
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