Comparative analysis of molecular activity in dermal mesenchymal stem cells from different passages
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Comparative analysis of molecular activity in dermal mesenchymal stem cells from different passages Xinhua Li . Junqin Li . Xincheng Zhao . Qiang Wang . Xiaohong Yang . Yueai Cheng . Min Zhou . Gang Wang . Erle Dang . Xiaoli Yang . Ruixia Hou . Peng An . Guohua Yin . Kaiming Zhang
Received: 26 June 2017 / Accepted: 15 November 2017 Ó Springer Science+Business Media B.V., part of Springer Nature 2017
Abstract Mesenchymal stem cells (MSCs) are used for tissue regeneration in several pathological conditions, including autoimmune diseases. However, the optimal sources and culture requirements for these cells are still under investigation. Here, we compared mRNA expression in dermal MSCs (DMSCs) at passage (P) 3 and P5 to provide a reference for future studies related to DMSCs expansion. In normal DMSCs, the expression of three of eight genes associated with basic cellular activity were different at P5 compared to that at P3: PLCB4 and SYTL2 were upregulated by 4.30- and 6.42-fold, respectively (P \ 0.05), whereas SATB2 was downregulated by 39.25-fold (P \ 0.05). At the same time, genes associated with proliferation, differentiation, inflammation, and apoptosis were expressed at similar levels at P3 and P5 (P [ 0.05). In contrast, in DMSCs isolated from psoriatic patients we observed differential expression of three inflammation-associated genes X. Li J. Li X. Zhao Q. Wang X. Yang Y. Cheng M. Zhou X. Yang R. Hou P. An G. Yin K. Zhang (&) Shanxi Key Laboratory of Stem Cell for Immunological Dermatosis, Institute of Dermatology, Taiyuan City Center Hospital, No. 1 Dong San Dao Xiang, Jiefang Road, Taiyuan 030009, Shanxi Province, China e-mail: [email protected] G. Wang E. Dang Hospital of Xijing Dermatology, Xijing Hospital, No. 15 Changle Road West, Xi’an 710032, Shanxi Province, China
at P5 compared to P3; thus IL6, IL8, and CXCL6 mRNA levels were upregulated by 16.02-, 31.15-, and 15.04-fold, respectively. Our results indicate that normal and psoriatic DMSCs showed different expression patterns for genes related to inflammation and basic cell activity at P3 and P5, whereas those for genes linked to proliferation, differentiation, and apoptosis were mostly similar. Keywords Culture Dermal mesenchymal stem cells mRNA expression Passage
Introduction More than 40 years ago, Friedenstein et al. (1968) first isolated fibroblast-like cells from bone marrow by exploiting their inherent property of adhering to plastic culture dishes; these cells were later designated as bone marrow-derived mesenchymal stem cells (MSCs). MSCs are primitive cells originated from the mesodermal germ layer and characterized by the ability to differentiate into various cell types, giving rise to connective tissue, skeletal muscle, and the vascular system. After isolation from bone marrow, MSCs were also obtained from other tissues and organs, including umbilical cord blood, adipose tissue, the lung, and skin.
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Cell Tissue Bank
Mesenchymal stem cells (MSCs) are typically purified by adherence to plastic an
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