Dissimilar characteristics of umbilical cord mesenchymal stem cells from donors of different ages
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Dissimilar characteristics of umbilical cord mesenchymal stem cells from donors of different ages Sha Huang • Changjiang Feng • Yan Wu • Siming Yang • Kui Ma • Xu Wu • Xiaobing Fu
Received: 25 September 2012 / Accepted: 22 January 2013 Ó Springer Science+Business Media Dordrecht 2013
Abstract Increasing attention is being placed on the use of adult stem cells due to their potential therapeutic applications for unmet medical needs. Among all adult stem cell types, umbilical cord mesenchymal stem cells (UCMSCs) can be collected easily and does not harm newborns and the mothers. The excellent pluripotency and proliferation potential of UCMSCs has been previously demonstrated by many groups. However, little is known about the different characteristics of these cells due to diversity of donors (e.g., the mothers’ age). Regarding this, we examined the effect of mothers’ age-related variation on the basic properties (proliferation, colony forming and
Sha Huang and Changjiang Feng contributed equally to this work. S. Huang X. Fu Wound Healing and Cell Biology Laboratory, Institute of Basic Medical Sciences, General Hospital of PLA, Beijing 100853, People’s Republic of China S. Huang C. Feng Y. Wu S. Yang K. Ma X. Fu (&) Burns Institute, The First Affiliated Hospital, General Hospital of PLA, Trauma Center of Postgraduate Medical College, 51 Fu Cheng Road, Beijing 100048, People’s Republic of China e-mail: [email protected]; [email protected]
differentiation potential) of UCMSCs in vitro. The data evidenced that elder mother results in reduction of both proliferative and colony forming capacity of UCMSCs, and impacts proliferative potential more significantly. Data also evidenced that a decline in osteogenic potential, but an increase in adipogenic potential of UCMSCs from the elder donors. Additionally, no phenotypic differences were observed by flow cytometry analysis using a panel of 6 main surface antigen markers. We observed no differences in karyotyping and both UCMSCs populations exhibited diploid cells without chromosomal aberrations. The results of our study are the basis for banking UCMSCs, which may be labeled with donors’ information for different experiments, and the mother age-related differences in characteristics of UCMSCs
Y. Wu Mudanjing Medical College, Mudanjing 157011, People’s Republic of China X. Wu Department of Thoracic and Cardiovascular Surgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, Guangdong Province, People’s Republic of China
C. Feng X. Wu Southern Medical University, Guangzhou 510515, People’s Republic of China
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should be taken into account when these cells are considered for further practical applications. Keywords Characteristics Umbilical cord Mesenchymal stem cells Donor Age
Introduction Stem cell research is likely to offer the opportunity to impact the treatment of patients, either to improve on already available therapy or to address previously unmet clinical needs. Currently, many di
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