Mesenchymal Stem Cells Methods and Protocols
This volume aims to outline the current status of the Mesenchymal Stem Cells(MSC) field in regenerative medicine and to propose clear and reproducible protocols to better define the identity, function and use of these cells that are today, more than
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Massimiliano Gnecchi Editor
Mesenchymal Stem Cells Methods and Protocols Second Edition
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Mesenchymal Stem Cells Methods and Protocols Second Edition
Edited by
Massimiliano Gnecchi Department of Molecular Medicine, Unit of Cardiology, University of Pavia, Pavia, Italy Department of Cardiothoracic and Vascular Sciences – Coronary Care Unit and Laboratory of Clinical and Experimental Cardiology, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy Laboratory of Experimental Cardiology for Cell and Molecular Therapy, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy Department of Medicine, University of Cape Town, Cape Town, South Africa
Editor Massimiliano Gnecchi Department of Molecular Medicine Unit of Cardiology University of Pavia Pavia, Italy Department of Cardiothoracic and Vascular Sciences – Coronary Care Unit and Laboratory of Clinical and Experimental Cardiology Fondazione IRCCS Policlinico San Matteo Pavia, Italy Laboratory of Experimental Cardiology for Cell and Molecular Therapy Fondazione IRCCS Policlinico San Matteo Pavia, Italy Department of Medicine University of Cape Town Cape Town, South Africa
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-3582-6 ISBN 978-1-4939-3584-0 (eBook) DOI 10.1007/978-1-4939-3584-0 Library of Congress Control Number: 2016936658 © Springer Science+Business Media New York 2008, 2016 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. Printed on acid-free paper This Humana Press imprint is published by Springer Nature The registered company is Springer Science+Business Media LLC New York
Preface Mesenchymal stem cells (MSC) are adult cells with the capacity for self-renewal and multilineage differentiation. Initially described in the bone marrow, MSC are also present
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