Comparative analysis of transcriptomes revealed the molecular mechanism of development of Tricholoma matsutake at differ
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Comparative analysis of transcriptomes revealed the molecular mechanism of development of Tricholoma matsutake at different stages of fruiting bodies Xian Tang1 • Xiang Ding2 • Yi-ling Hou1
Received: 21 August 2019 / Revised: 17 December 2019 / Accepted: 2 January 2020 The Korean Society of Food Science and Technology 2020
Abstract The purpose of the study is to investigate the molecular mechanisms of development of Tricholoma matsutake fruiting body at the primordial stage (TM-1), the intermediate stage (TM-2) and the mature stage (TM-3) using RNA-Seq sequencing technology. The analysis of gene expression level revealed that the Spn2 and Eef1a1 gene were the key genes in the primordial stage of T. matsutake by regulating cytokinesis, protein synthesis, and cell growth. And the Ubc, Atp6, Cytb, and Pth2 gene were the key genes in the mature stage of T. matsutake by regulating energy metabolism and protein synthesis. Differential expression genes (DEGs) analysis results showed that Cdc28, Rad53, Dun1, Pho85 and Pho81 were the key DEGs regulating cell cycle genes of T. matsutake from primordial stage to intermediate stage. And APC, Cyr1, Cdc45, Spo11 and Rec8 genes were the key DEGs for the meiosis and sporogenesis of T. matsutake from the intermediate stage to the mature stage.
& Yi-ling Hou [email protected] Xian Tang [email protected] Xiang Ding [email protected] 1
Key Laboratory of Southwest China Wildlife Resources Conservation (Ministry of Education), College of Life Sciences, China West Normal University, 1# Shida Road, Nanchong 637009, Sichuan Province, China
2
College of Environmental Science and Engineering, China West Normal University, 1# Shida Road, Nanchong 637009, Sichuan Province, China
Keywords Tricholoma matsutake Molecular mechanism Development RNA-sequencing Transcriptome
Introduction The molecular mechanism of the formation and development of edible fungus fruiting body is critical to their yield and quality. It is a complex process for the fruiting bodies of edible fungi differentiate and develop. With the rapid development of transcriptome sequencing technology, high-throughput sequencing has been used for transcriptome analyses of mushrooms, such as Lentinula edodes (Wang et al., 2018), Agrocybe aegerita (Wang et al., 2013), Pleurotus eryngii (Xie et al. 2018), Cordyceps guangdongensis (Zhang et al., 2018) and Coprinopsis cinerea (Plaza et al., 2014), and the molecular mechanism of macrofungus development has gradually been revealed. At the same time, some genes and transcription factors related to the growth and development of fruiting bodies of macrofungus have been excavated. For instance, differential expression of fvh1 (Ando et al., 2001), noxs (Mu et al., 2014), hsp9 (Kurahashi et al., 2014), dst (Kuratani et al., 2010; Terashima et al., 2005), eln3 (Arima et al., 2004), ppo (Li et al., 2011) and pro1 (Steffens et al., 2016) were found in the fruiting body of macrofungus formation. Tricholoma matsutake is an ectomycorrhizal fungus which belongs to the subgenus Tricholo
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