Comparative transcriptome analysis revealed resistance differences of Cavendish bananas to Fusarium oxysporum f.sp. cube
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RESEARCH ARTICLE
Open Access
Comparative transcriptome analysis revealed resistance differences of Cavendish bananas to Fusarium oxysporum f.sp. cubense race1 and race4 Honghong Dong, Yiting Ye, Yongyi Guo and Huaping Li*
Abstract Background: Banana Fusarium wilt is a devastating disease of bananas caused by Fusarium oxysporum f. sp. cubense (Foc) and is a serious threat to the global banana industry. Knowledge of the pathogenic molecular mechanism and interaction between the host and Foc is limited. Results: In this study, we confirmed the changes of gene expression and pathways in the Cavendish banana variety ‘Brazilian’ during early infection with Foc1 and Foc4 by comparative transcriptomics analysis. 1862 and 226 differentially expressed genes (DEGs) were identified in ‘Brazilian’ roots at 48 h after inoculation with Foc1 and Foc4, respectively. After Foc1 infection, lignin and flavonoid synthesis pathways were enriched. Glucosinolates, alkaloidlike compounds and terpenoids were accumulated. Numerous hormonal- and receptor-like kinase (RLK) related genes were differentially expressed. However, after Foc4 infection, the changes in these pathways and gene expression were almost unaffected or weakly affected. Furthermore, the DEGs involved in biological stress-related pathways also significantly differed after infection within two Foc races. The DEGs participating in phenylpropanoid metabolism and cell wall modification were also differentially expressed. By measuring the expression patterns of genes associated with disease defense, we found that five genes that can cause hypersensitive cell death were upregulated after Foc1 infection. Therefore, the immune responses of the plant may occur at this stage of infection. Conclusion: Results of this study contribute to the elucidation of the interaction between banana plants and Foc and to the development of measures to prevent banana Fusarium wilt. Keywords: Brazilian, Fusarium oxysporum f. sp. cubense, RNA-seq, Interaction, Resistance differences
Background Banana (Musa spp.) is among the paramount fruit corps worldwide due to its high nutritive value [1]. Banana Fusarium wilt, which is caused by Fusarium oxysporum f. sp. cubense (Foc), is one of the most destructive plant diseases that affects banana production worldwide. It has * Correspondence: [email protected] Guangdong Province Key Laboratory of Microbial Signals and Disease Control, College of Agriculture, South China Agricultural University, Guangzhou 510642, China
led to considerable production losses since it was first discovered in Australia in 1876 [2]. Foc has been divided into three physiological races, race1 (Foc1), race2 (Foc2), and race4 (Foc4), on the basis of host type and pathogenicity. These races attack different banana cultivars. Foc1 and Foc4 are the most important races owing to their extensive distribution in South China and considerable yield loss. The Foc1 resistant cultivar ‘Cavendish’ (AAA) subgroup has dominated banana exports since the 1960s,
© The Author(s). 2020 Open Access T
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