Comparative Leaf Proteomics of Brassica napus Genotypes with Distinctive Levels of Early Cold Acclimation
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ORIGINAL ARTICLE
Comparative Leaf Proteomics of Brassica napus Genotypes with Distinctive Levels of Early Cold Acclimation Khazar Edrisi Maryan 1,2 & Habibollah Samizadeh Lahiji 2 & Naser Farrokhi 1 Hassan Hasani Komeleh 2
&
Paul A. Haynes 3 & Sara Hamzelou 3 &
Received: 18 April 2020 / Accepted: 22 September 2020 # Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract Rapeseed/canola (Brassica napus L.) is considered amongst the “big four” oilseed crops with varying degrees of cold tolerance. Differential protein expression of cold acclimated young leaves of two canola genotypes, Sari Gul (spring type cold-sensitive) and Zarfam (winter type cold-tolerant), were determined via two dimensional polyacrylamide gel electrophoresis. Seedling leaves were cold acclimated by temperature regime and collected before and after cold treatment at 4 and − 4 °C after 24 h, proteins were isolated and separated on 2-DE. Gel analysis of 651 protein spots revealed that 19 protein spots were significantly different between the two conditions in response to early cold acclimation. Following nanoflow liquid chromatography–tandem mass spectrometry, 19 protein sequences were identified in gel spots, which were reported for the first time in response to early cold acclimation in B. napus and nearly half of them were associated with various aspects of chloroplast physiology; suggesting that the cold stress response of B. napus is achieved, at least partly, by regulation of chloroplast function. Our work has provided novel insights into the plant response to cold stress and should pave the way for future studies towards functional analysis of candidate genes in cold response. Keywords Canola . Cold acclimation . 2-dimensional electrophoresis . Peptide-to-spectrum matching Key Message • Cold acclimation is different between the cold tolerant vs cold sensitive genotypes that can be seen through changes of protein profiles. • The highly affected cellular organelle in response to cold stress is chloroplast as reflected in expression changes that are reported in the corresponding proteins. • Cold stress has pronounced effects on metabolic pathways. • The expressions of corresponding genes to changed proteins appear to be in control of plant hormones, mainly methyl jasmonate and abscisic acid as determined via analysis of cis-elements within the promoters. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s11105-020-01249-4) contains supplementary material, which is available to authorized users. * Habibollah Samizadeh Lahiji [email protected] * Naser Farrokhi [email protected]
Hassan Hasani Komeleh [email protected]
1
Department of Cell and Molecular Biology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Evin, Tehran, Iran
2
Department of Plant Biotechnology, Faculty of Agriculture, University of Guilan, Rasht, Iran
3
Department of Molecular Sciences, Macquarie University, North Ryde, NSW 2109, Australia
Khazar Edrisi Maryan kh.edri
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