Comparative metabolomics of Phialemonium curvatum as an omnipotent fungus cultivated on crude palm oil versus glucose
- PDF / 2,377,356 Bytes
- 16 Pages / 595.276 x 790.866 pts Page_size
- 98 Downloads / 164 Views
Microbial Cell Factories Open Access
RESEARCH
Comparative metabolomics of Phialemonium curvatum as an omnipotent fungus cultivated on crude palm oil versus glucose Arief Izzairy Zamani1, Susann Barig2, Sarah Ibrahim1, Hirzun Mohd. Yusof3, Julia Ibrahim3, Jaime Yoke Sum Low3, Shwu Fun Kua3, Syarul Nataqain Baharum1*, Klaus‑Peter Stahmann2* and Chyan Leong Ng1*
Abstract Background: Sugars and triglycerides are common carbon sources for microorganisms. Nonetheless, a systematic comparative interpretation of metabolic changes upon vegetable oil or glucose as sole carbon source is still lack‑ ing. Selected fungi that can grow in acidic mineral salt media (MSM) with vegetable oil had been identified recently. Hence, this study aimed to investigate the overall metabolite changes of an omnipotent fungus and to reveal changes at central carbon metabolism corresponding to both carbon sources. Results: Targeted and non-targeted metabolomics for both polar and semi-polar metabolites of Phialemonium curvatum AWO2 (DSM 23903) cultivated in MSM with palm oil (MSM-P) or glucose (MSM-G) as carbon sources were obtained. Targeted metabolomics on central carbon metabolism of tricarboxylic acid (TCA) cycle and glyoxylate cycle were analysed using LC–MS/MS-TripleQ and GC–MS, while untargeted metabolite profiling was performed using LC– MS/MS-QTOF followed by multivariate analysis. Targeted metabolomics analysis showed that glyoxylate pathway and TCA cycle were recruited at central carbon metabolism for triglyceride and glucose catabolism, respectively. Signifi‑ cant differences in organic acids concentration of about 4- to 8-fold were observed for citric acid, succinic acid, malic acid, and oxaloacetic acid. Correlation of organic acids concentration and key enzymes involved in the central carbon metabolism was further determined by enzymatic assays. On the other hand, the untargeted profiling revealed seven metabolites undergoing significant changes between MSM-P and MSM-G cultures. Conclusions: Overall, this study has provided insights on the understanding on the effect of triglycerides and sugar as carbon source in fungi global metabolic pathway, which might become important for future optimization of car‑ bon flux engineering in fungi to improve organic acids production when vegetable oil is applied as the sole carbon source. Keywords: Phialemonium curvatum, Omnipotent fungus, Comparative metabolomics, Central carbon metabolism, Selective minimal media
*Correspondence: [email protected]; Klaus‑Peter.Stahmann@b‑tu.de; [email protected] 1 Institute of Systems Biology, Universiti Kebangsaan Malaysia (UKM), 43600 Bangi, Selangor, Malaysia 2 Institute of Biotechnology, Brandenburg University of Technology Cottbus –Senftenberg, Universitaetsplatz 1, 01968 Senftenberg, Germany Full list of author information is available at the end of the article
Background Glucose is a common carbon source in fermentation. Recently, several studies have been done on plant triglycerides or vegetable oil to replace glucose in fermentation [1–5]. Fo
Data Loading...
