Comparing bone tissue engineering efficacy of HDPSCs, HBMSCs on 3D biomimetic ABM-P-15 scaffolds in vitro and in vivo
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ORIGINAL ARTICLE
Comparing bone tissue engineering efficacy of HDPSCs, HBMSCs on 3D biomimetic ABM-P-15 scaffolds in vitro and in vivo Yamuna Mohanram . Jingying Zhang . Eleftherios Tsiridis . Xuebin B. Yang
Received: 6 April 2020 / Accepted: 19 July 2020 Ó The Author(s) 2020
Abstract Human bone marrow mesenchymal stem cells (HBMSCs) has been the gold standard for bone regeneration. However, the low proliferation rate and long doubling time limited its clinical applications. This study aims to compare the bone tissue engineering efficacy of human dental pulp stem cells (HDPSCs) with HBMSCs in 2D, and 3D anorganic bone mineral (ABM) coated with a biomimetic collagen peptide (ABM-P-15) for improving boneforming speed and efficacy in vitro and in vivo. The multipotential of both HDPSCs and HBMSCs have been compared in vitro. The bone formation of HDPSCs on ABM-P-15 was tested using in vivo model. The osteogenic potential of the cells was confirmed by alkaline phosphatase (ALP) and immunohistological staining for osteogenic markers. Enhanced ALP, collagen, lipid droplet, or
Y. Mohanram J. Zhang X. B. Yang (&) Biomaterials & Tissue Engineering Group, Department of Oral Biology, School of Dentistry, University of Leeds, Level 7, Wellcome Trust Brenner Building, St. James’s University Hospital, Leeds LS9 7TF, UK e-mail: [email protected] J. Zhang The Second Clinical Medical College, Guangdong Medical University, Dongguan 523808, Guangdong, China E. Tsiridis Academic Orthopaedic Department, Aristotle University Medical School, 54124 Thessaloniki, Greece
glycosaminoglycans production were visible in HDPSCs and HBMSCs after osteogenic, adipogenic and chondrogenic induction. HDPSC showed stronger ALP staining compared to HBMSCs. Confocal images showed more viable HDPSCs on both ABM-P-15 and ABM scaffolds compared to HBMSCs on similar scaffolds. ABM-P-15 enhanced cell attachment/ spreading/bridging formation on ABM-P-15 scaffolds and significantly increased quantitative ALP specific activities of the HDPSCs and HBMSCs. After 8 weeks in vivo implantation in diffusion chamber model, the HDPSCs on ABM-P-15 scaffolds showed extensive high organised collagenous matrix formation that was positive for COL-I and OCN compared to ABM alone. In conclusion, the HDPSCs have a higher proliferation rate and better osteogenic capacity, which indicated the potential of combining HDPSCs with ABM-P-15 scaffolds for improving bone regeneration speed and efficacy. Keywords PepGen P-15 HDPSCs HBMSCs Bone tissue engineering In vivo
Introduction The increasing clinical demand for bone regeneration and repair in the context of our ageing population poses a challenge both to healthcare providers and
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society (Iaquinta et al. 2019). There is also increasing demand for the implant osseointegration, which is crucial for successful implantology in both orthopaedics and dentistry (Chandran and John 2019; Liu et al. 2019). Tissue engineering provides a promising strateg
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