Comparison of automated and conventional microbiological examination of donated human cardiac tissue in heart valve bank
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Comparison of automated and conventional microbiological examination of donated human cardiac tissue in heart valve banking Letı´cia Kraft . Felipe Francisco Tuon . Victoria Stadler Tasca Ribeiro . Luciana Cristina Wollmann . Paula Hansen Suss . Marcelo Pilonetto . Francisco Diniz Affonso da Costa
Received: 11 January 2018 / Accepted: 17 April 2018 Ó Springer Science+Business Media B.V., part of Springer Nature 2018
Abstract Most tissue banks use the conventional method; however, the automated method has advantages over the conventional method. The aim of this study was to compare the conventional and automated methods of culture in human cardiac tissue using an artificial contamination model. Myocardial samples were contaminated with sequential concentration (104 to 10-1 CFU/mL) with Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus and Candida albicans. Cultures were obtained from solution were the fragment was immersed and minced tissue, before and after the routine decellularization solution, with automated and conventional culture methods. Automated and conventional methods were compared and a p value B 0.05 was considered significant. Staphylococcus aureus presented a significantly higher growth in the automated method, as well as faster than the conventional (p \ 0.05). The positivity for growth in the automated method was higher in concentrated
All authors meet the ICMJE authorship criteria. L. Kraft F. F. Tuon V. S. T. Ribeiro L. C. Wollmann P. H. Suss F. D. A. da Costa Human Bank Tissue, Pontifı´cia Universidade Cato´lica do Parana´, Curitiba, PR 80215-901, Brazil F. F. Tuon (&) M. Pilonetto School of Medicine, Pontifı´cia Universidade Cato´lica do Parana´, Rua Imaculada Conceic¸a˜o 1155, Curitiba, PR 80215-901, Brazil e-mail: [email protected]
inoculum ([ 102 CFU/mL) (p \ 0.05). The growth in the automated method was significantly faster than conventional when inoculum concentration was above 103 CFU/mL. The automated culture method is faster than conventional method with a higher positivity in a contaminated model of myocardial and transport solution used in tissue banks. Keywords Blood culture Automated method Tissue bank Valves
Introduction Allograft heart valves have been successfully applied in reconstructive surgery of acquired and congenital heart defects (Cebotari et al. 2011) and continue to represent almost perfect substitutes (Lisy et al. 2017). However, microbiological contamination of retrieved tissues is a critical aspect in the safety of human tissues intended for clinical use (Paolin et al. 2017). The contamination of heart valves can occur in different steps, such as donor hidden infection, contamination of the environment, materials used during organ explantation and ex vivo manipulation (Kowalski et al. 2012a, b). Currently, tissue banks worldwide have methodological differences regarding microbiological control. During the decontamination procedures of valve tissues, protocols differ mainly in composition and
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