Comparison Study on the Biomass Recalcitrance of Different Tissue Fractions of Sugarcane Culm
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Comparison Study on the Biomass Recalcitrance of Different Tissue Fractions of Sugarcane Culm Michel Brienzo & Solange Ferreira & Marcos P. Vicentim & Wanderley de Souza & Celso Sant’Anna
# Springer Science+Business Media New York 2014
Abstract Conversion of sugarcane bagasse to bioethanol is hindered due to intrinsic biomass recalcitrance that is related to its chemical composition and physicochemical properties. Furthermore, biomass heterogeneity interferes with process effectiveness. To compare sugarcane culm recalcitrance epidermis, internode and node fractions were individually investigated by acid pretreatment, enzymatic hydrolysis and thermal analysis/degradation. The epidermis fraction was found to be the largest mass fraction of the sucrose-free sugarcane culm followed by the epidermis-free internode and node fractions, comprising 65, 19 and 15.5 %, respectively. In comparison to the internode and node acid pretreatment solubilized a lower level of xylose from the epidermis resulting in higher mass recovery of water-insoluble solids (WIS) demonstrating its higher resistance to acid pretreatment. Enzymatic digestion Electronic supplementary material The online version of this article (doi:10.1007/s12155-014-9487-8) contains supplementary material that is available to authorized users. M. Brienzo (*) : S. Ferreira : W. de Souza : C. Sant’Anna Laboratory of Structural Biology, Metrology Applied to Science life– National Institute of Metrology, Quality and Technology (Inmetro), Av. Nossa Senhora das Graças, 50–Xerém, CEP 25,250-020 Duque de Caxias, RJ, Brazil e-mail: [email protected] M. Brienzo e-mail: [email protected] M. P. Vicentim Laboratory of Organic Analysis, Division of Chemical Metrology– National Institute of Metrology, Quality and Technology (Inmetro), Av. Nossa Senhora das Graças, 50–Xerém, CEP 25,250-020 Duque de Caxias, RJ, Brazil W. de Souza Laboratory of Cell Structure, Institute of Biophysics Carlos Chagas Filho, National Science and Technology Institute in Structural Biology and Bioimaging, Rio de Janeiro Federal University, Rio de Janeiro, RJ, Brazil
showed that the epidermis is least susceptible to hydrolysis followed by the node and internode: 18.6, 56.5 and 75.9 %, respectively. In agreement with the enzymatic hydrolysis yield the internal/external surface area was lower for the epidermis than for the node and internode. Scanning electron microscopy (SEM) showed the epidermis exhibited less structural damage after enzymatic hydrolysis. Moreover, the epidermis required a higher start temperature for degradation (330 °C) and exhibited a higher heating value (4,236 cal/g). The internode and node required a degradation start temperature of 288 and 265 °C and had heating values of 4,098.9 and 3,998.76 cal/g, respectively. Taken together the results of this study demonstrate that the epidermis is more resistant to pretreatment, to thermal and enzyme degradation than are the internode and node. The separation of the epidermis from the culm could provide a new perspective on pr
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