Comparison with Amino Group and Hydrophilic Group for Protein Afftnity by Excimer Laser Induced Functional Groups Substi
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C11.18.1
COMPARISON WITH AMINO GROUP AND HYDROPHILIC GROUP FOR PROTEIN AFFINITY BY EXCIMER LASER INDUCED FUNCTIONAL GROUPS SUBSTITUTION ONTO PET FILM Hitoshi Omuro, Masato Nakagawa*, Hiroaki Fukuda** and Masataka Murahara Department of Electrical Engineering, Tokai University 1117 Kitakaname, Hiratuka-shi, Kanagawa 259-1292, Japan *Mizue Clinic, 3-6-95 Nisimizue, Edogawa, Tokyo 132-0015, Japan **Saiseikai Hiratuka Hospital, 37-1 Tatino, Hiratuka, Kanagawa 254, Japan ABSTRACT PET has been widely used for medical materials such as an artificial ligament. However, the affinity of tissues is no good. To compensate this, the mesh formed PET has clinically been used for artificial ligament intrud ing tissue into mesh. However, this method has not shown sufficient affinity with the tissue; that is, the initial adapting strength of the material and tissue is weak. The artificial ligament must be biocompatible to contact blood and tissue. The foregoing artificial ligament, however, doesn’t satisfy the biocompatibility. Thus, we have modified the PET surface into hydrophilic by substituting NH2 or OH functional groups. Firstly, an ArF excimer laser light was irradiated the PET with water on top. The OH functional group was substituted on the PET surface by this photochemical reaction. Secondly, the ArF excimer laser light was irradiated the PET in ammonia gas ambient. In this photochemical reaction, the NH2 functional group was substituted on the PET surface. In this study, the untreated sample had the contact angle with water of 80 degrees and the bonding strength with protein of only 1.0kg/cm2 . The contact angle of the modified sample improved to 40 degrees and the bonding strength, to 23kg/cm2 . When treated in ammonia gas, the contact angle also improved to 40 degrees; however, the bonding strength was almost the same as that of the untreated sample. It was confirmed that the affinity of the PET for water and protein could be controlled by increasing or decreasing the substitution concentration of OH and NH2 functional groups on the surface. INTRODUCTION The number of patients with ruptured ligament is increasing by accidents of sports and traffic. Therefore, an artificial ligament has been developed. The current artificial ligaments clinically applied are the ligament twisted from drawing processed Teflon [1], Kennedy’s LAD (ligament augmentation device) made of polypropylene fiber [2], Leeds-Keio artificial ligament developed by Leeds University and Keio University and made of polyester mesh [3], and others. The artificial ligament must be biocompatible in addition to its functional and dynamic characteristics because it directly contacts blood and tissues. The foregoing artificial ligaments satisfy the two characteristics of material function and dynamics, but the biocompatibility is not satisfactory. It is critical for not the bulk but the material surface to be biocompatible for clinical application. Therefore, the surface modification of various materials has been studied extensively.
C11.18.2
Tomoko Ueda-Yukos
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