Comparison of -OH and -NH 2 Functional Group Substitution on PTFE Surface with V-UV Photon Irradiation for Protein Adsor
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Comparison of -OH and -NH2 Functional Group Substitution on PTFE Surface with V-UV Photon Irradiation for Protein Adsorption Yuji Sato, Naoki Kobayashi and Masataka Murahara Department of Electrical and Electronic Engineering, Tokai University 1117 Kitakaname Hiratsuka Kanagawa, 259-1292, JAPAN ABSTRACT Poly-tetrafluoroethylene [PTFE] presents few rejections in a living body but has low tissue affinity. Then, the soft tissue implant material that has not only high biocompatibility but also superb bondability has been developed by photo-chemically substituting the hydrophilic of –OH or –NH2 groups on the PTFE surface with V-UV photon irradiation. The protein adsorption of the sample before and after treatment was also evaluated by scanning electron microscope [SEM] and attenuated total reflection Fourier-transform infrared [ATR FT-IR], using bovine serum albumin [ALB] and fibrin [FIB] solution as a protein index in biocompatibility test. From the results, it has been confirmed that the protein adsorption increased with the increase in the hydrophilic group’s substitution density. The -OH incorporated sample adsorbed the ALB and FIB more than the -NH2 incorporated sample; the amount of the ALB and FIB sticking became 2.3 times larger than that of the non-treatment sample. INTRODUCTION In general, when a plastic material is implanted in a human body, proteins are adsorbed on the plastic surface; on which a fibroblast or a tissue cell adheres to form a fibrous tissue. It is, therefore, essential for a biomaterial to be biocompatible, chemically resistant and have resistance to hostile environments. However, many plastics, which have been clinically employed as a soft tissue implant material, have low adhesiveness and affinity to tissue because of its low surface energy. In order to improve its adhesive property, Okada and Ikada reported that the carboxyl groups in poly (acrylic acid) chains were grafted onto the silicone surface, and the collagen was able to immobilize there [1]. M.C.L. Martins et al. produced a poly(hydroxyethylmethacrylate) (PHEMA) and a poly(hydroxyethylacrylate)(PHEA) film by the graft polymerization onto the poly(etherurethane)(PU) films to absorb the ALB and fibrinogen [2]. Satriano and Marletta + incorporated oxygen groups on a PHMS surface by O2 -plasma and Ar -ion beam irradiations and cultivated fibroblast on the modified surface [3]. Lee et al. formed the micropatterning on the oxygen plasma treated polystyrene surface by using KrF excimer laser ablation method and cultivated the NG 108-15 neuroblastoma x glioma hybrid cells [4]. They reported qualitatively that the protein adsorption depends on the functional groups and shape on the material surface.
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There are, however, no previous reports to investigate quantitatively relationship between protein adsorption and the functional groups or its density of material surface. Thus, we photo-chemically incorporated the hydrophilic groups on the PTFE surface, which has the most water repellency and causes only a few rejections in
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