Complete genome sequences of two novel dicistroviruses detected in yellow crazy ants ( Anoplolepis gracilipes )
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Complete genome sequences of two novel dicistroviruses detected in yellow crazy ants (Anoplolepis gracilipes) Chih‑Chi Lee1 · Chun‑Yi Lin2 · Hung‑Wei Hsu1 · Chin‑Cheng Scotty Yang2,3,4 Received: 16 April 2020 / Accepted: 5 July 2020 © Springer-Verlag GmbH Austria, part of Springer Nature 2020
Abstract We report two novel RNA viruses from yellow crazy ants, (Anoplolepis gracilipes) detected using next-generation sequencing. The complete genome sequences of the two viruses were 10,662 and 8,238 nucleotides in length, respectively, with both possessing two open reading frames with three conserved protein domains. The genome organization is characteristic of members of the genus Triatovirus in the family Dicistroviridae. The two novel viruses were tentatively named “Anoplolepis gracilipes virus 1” and “Anoplolepis gracilipes virus 2” (AgrV-1 and AgrV-2). Phylogenetic analyses based on amino acid sequences of the non-structural polyprotein (ORF1) suggest that the two viruses are triatovirus-like viruses. This is the first report on the discovery of novel triatovirus-like viruses in yellow crazy ants with a description of their genome structure (two ORFs and conserved domains of RNA helicase, RNA-dependent RNA polymerase, and capsid protein), complete sequences, and viral prevalence across the Asia-Pacific region. The high-throughput sequencing (HTS) technique has accelerated discovery of new viral pathogens in a number of arthropod taxa. The family Dicistroviridae includes RNA viruses with a linear, positive-sense, single-stranded genome that mainly infect arthropod hosts such as aphids, leafhoppers, flies, bees and ants [1]. The family Dicistroviridae includes three genera: Aparavirus, Cripavirus, and Triatovirus [1]. To date, seven triatovirus-like viruses have Handling Editor: Ana Cristina Bratanich. Chih-Chi Lee, Chun-Yi Lin and Hung-Wei Hsu contributed equally to this work. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00705-020-04769-2) contains supplementary material, which is available to authorized users. * Chin‑Cheng Scotty Yang [email protected]‑u.ac.jp 1
Laboratory of Insect Ecology, Graduate School of Agriculture, Kyoto University, Kyoto 606‑8502, Japan
2
Research Institute for Sustainable Humanosphere, Kyoto University, Kyoto 611‑0011, Japan
3
Department of Entomology, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA
4
Department of Entomology, National Chung Hsing University, Taichung 402204, Taiwan
been discovered, and form a monophyletic group with additinal five viruses of the genus Triatovirus, suggesting the existence of a total of 12 triatoviruses [2–6]. The majority of these triatoviruses were found to infect insects, including eight ant species [2–8]. While a previous study reported a partial sequence of an aparavirus-like virus in invasive yellow crazy ants (Anoplolepis gracilipes) [9], the diversity and prevalence of triatoviruses is largely unknown. Here, we rep
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