Complete genome sequence of a novel recombinant avian leukosis virus isolated from a three-yellow chicken
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Complete genome sequence of a novel recombinant avian leukosis virus isolated from a three‑yellow chicken Tao Sun1 · Ximei Wang2 · Wei Han1 · Xiaoling Ma5 · Weili Yin1 · Baohai Fang1 · Xiao Lin3 · Yang Li4 Received: 19 February 2020 / Accepted: 2 July 2020 © Springer-Verlag GmbH Austria, part of Springer Nature 2020
Abstract In this study, an avian leukosis virus (ALV) strain (GX-2020-01) was isolated from a three-yellow chicken, and its complete genome was 7570 bp long with the typical organization “5’LTR-gag-pol-env-3’LTR.” Phylogenetic analysis and sequence comparison revealed that it belongs to the ALV-J subgroup. However, the LTR region of GX-2020-01 is highly similar to that of reference strains of ALV-K/E (96.61%-97.10%), demonstrating that this novel isolate is a natural recombinant. The replication efficiency of GX-2020-01 was significantly lower than the previously isolated ALV-J strain (NX0101), indicating that the recombination event might have resulted in slower virus replication, making it harder for it to be detected through routine testing. Avian leukosis virus (ALV) belongs to the genus Alpharetrovirus of the family Retroviridae and induces the first known virus-related tumor disease in chickens [1]. To date, eleven subgroups of ALV (designated as ALV-A to ALV-K) have been identified based on differences in their host range, cross-neutralization patterns, and viral envelope interference Handling Editor: William G. Dundon. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00705-020-04764-7) contains supplementary material, which is available to authorized users. * Xiao Lin [email protected] * Yang Li [email protected] 1
Technical Center of Qingdao Customs, NO. 70 Qutangxia Road, Qingdao 266002, Shandong, China
2
Department of Breast Surgery, Qilu Hospital (Qingdao) of Shandong University, No. 758 Hefei Road, Shibei District, Qingdao 266000, Shandong, China
3
Nanning Mootek Intelligent Technology Co., Ltd, Yongfu Road, Yongning District, Nanning 530000, Guangxi, China
4
China Animal Health and Epidemiology Center, Qingdao 266000, China
5
Shanghai Pharmaceutical Group Qingdao Guofeng Pharmaceutical Co. LTD, NO.18 Songhuajiang Road, Economic and Technological Development Area, Qingdao 266000, China
[2, 3]. Members of subgroup J are the most pathogenic and have caused huge economic losses all over the world [4]. In China, ALV-J was first detected in 1999 and then spread rapidly throughout the country [5]. Fortunately, the nationwide eradication program implemented in China brought the virus under control, with fewer AL outbreaks in commercial broilers and layers after 2013 [6]. However, it is worth noting that some ALV-J field strains are still circulating in many local Chinese chicken breeds, such as three-yellow broilers [7], which provide a hotbed for the virus to persist and mutate [8–10]. Therefore, we need to pay more attention to the prevalence of ALV in indigenous chickens and monitor changes in its genome d
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