Comprehensive lipidomic profiling in serum and multiple tissues from a mouse model of diabetes
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ORIGINAL ARTICLE
Comprehensive lipidomic profiling in serum and multiple tissues from a mouse model of diabetes Zhen Chen1 · Qiangrong Liang2 · Yue Wu1 · Zijun Gao1 · Satoru Kobayashi2 · Joy Patel2 · Cairong Li3 · Fei Cai4 · Youhua Zhang2 · Chongsheng Liang1 · Hitoshi Chiba5 · Shu‑Ping Hui1 Received: 24 December 2019 / Accepted: 28 September 2020 © Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract Introduction Diabetes mellitus is a serious metabolic disorder causing multiple organ damage in human. However, the lipidomic profiles in different organs and their associations are rarely studied in either diabetic patients or animals. Objectives To evaluate and compare the characteristics of lipid species in serum and multiple tissues in a diabetic mouse model. Methods Semi-quantitative profiling analyses of intact and oxidized lipids were performed in serum and multiple tissues from a diabetic mouse model fed a high fat diet and treated with streptozotocin by using LC/HRMS and MS/MS. The total content of each lipid class, and the tissue-specific lipid species in all tissue samples were determined and compared by multivariate analyses. Results The diabetic mouse model displayed characteristic differences in serum and multiple organs: the brain and heart showed the largest reduction in cardiolipin, while the kidney had more alterations in triacylglycerol. Interestingly, the lipidomic differences also existed between different regions of the same organ: cardiolipin species with highly polyunsaturated fatty acyls decreased only in atrium but not in ventricle, while renal cortex showed longer fatty acyl chains for both increased and decreased triacylglycerol species than renal medulla. Importantly, diabetes caused an accumulation of lipid hydroperoxides, suggesting that oxidative stress was induced in all organs except for the brain during the development of diabetes. Conclusions These findings provided novel insight into the organ-specific relationship between diabetes and lipid metabolism, which might be useful for evaluating not only diabetic tissue injury but also the effectiveness of diabetic treatments. Keywords Lipidomics · Type 2 diabetes mellitus · Mouse model · Multiple tissues · Lipid hydroperoxides · Oxidative stress
1 Introduction
Zhen Chen and Qiangrong Liang contributed equally. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s11306-020-01732-9) contains supplementary material, which is available to authorized users. * Shu‑Ping Hui [email protected] 1
Faculty of Health Sciences, Hokkaido University, Kita‑12, Nishi‑5, Kita‑ku, Sapporo 060‑0812, Japan
2
Department of Biomedical Sciences, New York Institute of Technology College of Osteopathic Medicine, Old Westbury, NY 11548, USA
3
Clinical Medical College, Hubei University of Science and Technology, 437100 Xianning, China
Diabetes mellitus has become a serious metabolic disorder throughout the world, representing one of the leading causes of morbidity and
