Consolidated bioprocessing using an edible mushroom
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1219-AA01-06
Consolidated bioprocessing using an edible mushroom Ryoji Mizuno1 Hitomi Ichinose1 Tomoko Maehara1 Koji Takabatake2 and Satoshi Kaneko1 1 Food Biotechnology Division, National Food Research Institute, 2-1-12 Kannondai, Tsukuba, Ibaraki 305-8642, Japan 2 Foret Institute, Toyama Prefectural Agricultural, Forestry and Fisheries Research, 3 Yoshimine, Tateyama, Toyama 930-1362, Japan
ABSTRACT Worldwide attention has now focused on bioethanol production to combat global warming and to safeguard global energy. Lignocelluloses are expected to be utilized in future as fuel ethanol production because of competition between food and fuel production. One of the major problems in producing ethanol from lignocellulosic biomass is high production cost and consolidated bioprocessing (CBP) is gaining recognition as a potential breakthrough for low-cost biomass processing. Basidiomycetes appear suitable for use in CBP because they can achieve the both events of lignocellulose breakdown and ethanol fermentation. We are developing CBP bioethanol production by using Flammulina velutipes from sorghums. It turns out the relationship between varietal characteristics of sorghums and ethanol conversion properties of F. velutipes, and the direction should be performed in the future became clear.
INTRODUCTION Plant cell walls are the most abundant biomass source in nature and are of increasing importance because worldwide attention has now focused on bioethanol production to combat global warming and to safeguard global energy. Because of competition between food and fuel production, lignocelluloses are expected to be utilized in future fuel ethanol production. In spite of the many efforts, one of the major problems in producing ethanol from lignocellulosic biomass is high production cost. Recent technoeconomic analyses of the corn stover to ethanol process, currently based on dilute sulfuric acid and SSF (simultaneous saccharification and fermentation), report that the capital cost of pretreatment and production and/or purchase of cellulase enzymes remain the dominant cost hurdles to overcome [1,2]. Consolidated bioprocessing (CBP) is gaining recognition as a potential breakthrough for low-cost biomass processing, but no natural microorganism exhibits all the features desired for CBP [3,4]. CBP combines simultaneous saccharification of lignocellulose with fermentation of the resulting sugars into a single process step mediated by a single microorganism or microbial consortium. Compared to other less highly integrated configurations, CBP is distinctive because it does not contain a separate and dedicated process step for cellulase production [5]. In this study, we investigated the properties of ethanol fermentation by Flammulina velutipes to determine its suitability for CBP, because the use of basidiomycetes in bioethanol production is not common, and the ethanol fermentation abilities of basidiomycetes are not well characterized. Furthermore, the possibility of using two kinds of sorghum as raw materials in consolidated biop
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