Contribution of male genitors in open-pollination progenies of sour passion fruit
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Contribution of male genitors in open-pollination progenies of sour passion fruit Maria Helena Menezes Cordeiro . Claudio Horst Bruckner . Renato Domiciano Silva Rosado . Carlos Eduardo Magalha˜es dos Santos . Eveline Teixeira Caixeta . Tiago Vieira Sousa . Cosme Damia˜o Cruz
Received: 6 December 2019 / Accepted: 13 August 2020 Ó Springer Nature B.V. 2020
Abstract Passion fruit breeding programs often use a single fruit to obtain progeny. Open-pollination progenies are considered half-siblings since they allow the occurrence of a mixture of pollens during pollination. However, there are no studies able to prove that these progenies are, in fact, consequence of a mixture of pollens. The contribution of male genitors to the formation of open-pollinated progenies and genetic diversity were evaluated by means of microsatellite markers. A hybrid test was performed based on the gathered genotypic data, a chi-square test was used to verify the expected and observed frequencies, and the
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10681-020-02681-z) contains supplementary material, which is available to authorized users. M. H. M. Cordeiro (&) C. H. Bruckner R. D. S. Rosado C. E. M. dos Santos C. D. Cruz Department of Plant Science, Federal University of Vic¸osa, Av. Ph Rolfs S/N, Vic¸osa, Minas Gerais 365700-000, Brazil e-mail: [email protected] E. T. Caixeta Brazilian Agricultural Research Corporation - Embrapa Cafe´, BIOAGRO, BioCafe´, Federal University of Vic¸osa, Av. Ph Rolfs S/N, Vic¸osa, Minas Gerais 365700-000, Brazil T. V. Sousa Federal Institute of Goiano, Rod. Geraldo Silva Nascimento, Km 2.5, Urutaı´, Goia´s 75790-000, Brazil
proportion of identical genes by descent (IBD) was estimated. Genetic diversity was studied through descriptive analysis, cluster analysis, and molecular analysis (AMOVA). The hybrid test indicated the participation of more than one male genitor in progeny formation. However, the genotypic composition observed in the progenies differed significantly from that expected when assuming crosses between all individuals of the origin population. The IBD values for the progenies ranged from 0.26 to 0.30, indicating that they are progenies of half-siblings. The progenies showed observed heterozygosity greater than expected and an inbreeding coefficient close to zero. The AMOVA revealed that the greatest genetic variation (81.96%) occurred within progenies. In sour passion fruit, open-pollinated progenies obtained from a single fruit do not present genotypic composition representative of their population of origin since the greatest genetic diversity was observed within the progenies. Keywords Passiflora edulis Sims Genetic variability Half-sibling progenies Natural pollination
Introduction Passiflora edulis Sims, a species native to Brazil, belongs to the Passifloraceae family, and it is widely distributed throughout South and Central America
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