Analysis of the complete genome sequence of a potyvirus from passion fruit suggests its taxonomic classification as a me
- PDF / 904,498 Bytes
- 4 Pages / 595.276 x 790.866 pts Page_size
- 52 Downloads / 221 Views
ANNOTATED SEQUENCE RECORD
Analysis of the complete genome sequence of a potyvirus from passion fruit suggests its taxonomic classification as a member of a new species Ke Yang1 · Haixia Yan2 · Li Song3 · Pengfei Jin1 · Weiguo Miao1 · Hongguang Cui1 Received: 25 February 2018 / Accepted: 10 May 2018 © Springer-Verlag GmbH Austria, part of Springer Nature 2018
Abstract The complete genomic sequence of a telosma mosaic virus (TeMV) isolate (named PasFru), identified in passion fruit in China, was determined. The entire RNA genome of PasFru comprises 10,049 nucleotides (nt) excluding the poly(A) tail and encodes a polyprotein of 3,173 amino acids (aa), flanked by 5’ and 3’ untranslated regions (UTR) of 276 and 251 nt, respectively. Compared with the previous TeMV isolate Hanoi from Telosma cordata, the only documented isolate with the entire genome sequence annotated, PasFru had an extra 87 nt and 89 aa residues at the 3’-end of 5’UTR and the N-terminus of the P1 protein, respectively, which contributed to the genome size difference between PasFru and Hanoi (10,049 nt versus 9,689 nt). Pairwise sequence comparisons showed that PasFru shares 73.6% nt and 80.9% aa sequence identity with the Hanoi isolate at the whole-genome and polyprotein level, respectively, and these values are below the corresponding threshold values for species demarcation in the family Potyviridae. These data suggest that TeMV-PasFru should be classified as a new member of the genus Potyvirus. Telosma mosaic virus (TeMV) was first identified in Vietnam in Telosma cordata plants, and the corresponding isolate, named “Hanoi”, was completely sequenced and classified as a new member of the genus Potyvirus [1]. TeMV was subsequently detected in Indonesia in patchouli plants, using reverse transcription polymerase chain reaction (RT-PCR) detection of the coat protein (CP) gene [2]. Recently, TeMV was reported to infect passion fruit in Thailand, and the corresponding isolate (named Panda 15, GenBank accession Handling Editor: F. Murilo Zerbini. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00705-018-3885-8) contains supplementary material, which is available to authorized users. * Hongguang Cui [email protected] 1
College of Tropical Agriculture and Forestry, Hainan University, Haikou, Hainan 570228, People’s Republic of China
2
College of Forestry, Agricultural University of Hebei, Baoding, Hebei 071001, People’s Republic of China
3
College of International Cultural Exchange, Hainan University, Haikou, Hainan 570228, People’s Republic of China
no. AM409188) was judged to be a strain of TeMV, mainly due to the fact that its coat protein (CP) amino acid sequence shared 84% identity with that of the original TeMV isolate (Hanoi) from Telosma cordata, the only documented isolate with its entire genome sequence annotated [3]. In addition, an incomplete genome sequence of another TeMV isolate from passion fruit in China (GX1) is also available in the NCBI database (GenBank accession
Data Loading...
