Controlling Cell Growth by Nanoparticles
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0950-D04-15
Controlling Cell Growth by Nanoparticles Sergiy Zankovych1, Joerg Bossert1, Ines Thiele1, Klaus D. Jandt1, and Liga Berzina-Cimdina1,2 1 Institute for Materials Science and Technology, Friedrich-Schiller-University Jena, Loebdergraben 32, Jena, 07749, Germany 2 Biomaterials R & D Laboratory, Riga Technical University, 14/24 Azenes St., Riga, LV-1048, Latvia
ABSTRACT We report preliminary results of using nanoparticles to control cell attachment and growth. We present the way to create titanium surfaces with different roughness in a rage between 2 nm and 117 nm by using nanoparticles as a superficial layer and varying the evaporation parameters. We examined cell proliferation on titanium substrates with increased surface roughness compared to smooth titanium surface. We used nanoparticles to create a micrometer-sized lateral layout onto substrates preliminary structured by microcontact printing. We demonstrate controlled cell growth on substrates laterally structured with nanoparticles. INTRODUCTION The biocompatibility of biomaterials is closely related with the behaviour of cells at the cell/biomaterial interface. Initial cell attachment and spreading determines the ability of cells to proliferate and their long-term viability. Most important factors influencing the attachment, spreading, and proliferation of cells are surface topography [1-3], the surface energy and chemistry [4-6], and the surface roughness [7,8]. Increased proliferation of cells was observed on nano- or microstructured materials, such as nanoparticles or ceramics [2,3]. In this context, nanoparticles are attractive candidates for modifying biointerfaces. Particles with sub-micrometer diameters (nanoparticles) provide unique properties that are not available at the macroscopic scale. Nanoparticles can be found in daily use in a broad range of applications such as cosmetics and toothpastes, inks, food, and photo films, as well as in pharmaceutical and drug delivery applications. New and promising areas of application of nanoparticles are in the bio- and medical applications. In particular, such properties of nanoparticles as possessing high surface areas, varying chemical surface functionality, and binding of proteins or cells at the interface, coupled with their use for controlling the surface microstructure are of interest. As such, nanoparticles open a variety of possibilities to integrate special functions in biorelevant structures and coatings. Recently, TiO2 nanoparticles were used in layer-by-layer technique for controlling substrate surface roughness for increased stem cell proliferation [9]. The surface roughness was found to increase with increasing number of nanoparticle layers. The spreading of cells becomes faster, attachment and proliferation increase on surfaces with increasing number of nanoparticle layers. In this paper, we present the use of nanoparticles of different chemical compositions for the direct growth of cells, and also for their use as a superficial layer for depositing titanium films to create different
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