Corncob Residue Pretreatment for 2,3-Butanediol Production by Simultaneous Saccharification and Fermentation
In order to utilize corncob residue in the production of 2,3-butanediol, a pretreatment method was carried out. Sodium hydroxide method was chosen from six different types of pretreatment for lignocellulosic materials. After the composition analysis and f
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Corncob Residue Pretreatment for 2,3-Butanediol Production by Simultaneous Saccharification and Fermentation Xiaopei Peng, Cuiying Zhang, Yujie Tian, Xuewu Guo, Yanwen Liu and Dongguang Xiao Abstract In order to utilize corncob residue in the production of 2,3-butanediol, a pretreatment method was carried out. Sodium hydroxide method was chosen from six different types of pretreatment for lignocellulosic materials. After the composition analysis and fermentation experiment of the treated residue, the condition of pretreatment was suggested (hydroxide concentration of 1.5 %, processing temperature at 80 °C, processing time of 3 h, solid-to-liquid ratio of 1:8). Comparison between the primary material and the treated residue was conducted, the results proved that the output of 2,3-butanediol and acetoin (20.35 g/L) with the treated material was 3.38 times of the yield with the unpretreated corncob residue (6.02 g/L). After 72 h SSF, sugar yield of the cellulose was 75.40 %.
Keywords 2,3-Butanediol Corncob residue Lignocellulose Simultaneous saccharification and fermentation
Pretreatment
156.1 Introduction 2,3-Butanediol (2,3-BD) is a kind of transparent, odorless, and tasteless liquid. As an important platform chemical of the petroleum substitution theorem [1], its derivatives have a wide range of potential applications in many fields [2–5]. Recently, the production of 2,3-BD by microbial fermentation received increasing attention because of its economical efficiency [6–8]. However, the caring of the concentration of product and the neglecting of the raw material cost by most of the X. Peng C. Zhang (&) Y. Tian X. Guo Y. Liu D. Xiao Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin Industrial Microbiology Key Laboratory, College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, People’s Republic of China e-mail: [email protected]
T.-C. Zhang et al. (eds.), Proceedings of the 2012 International Conference on Applied Biotechnology (ICAB 2012), Lecture Notes in Electrical Engineering 251, DOI: 10.1007/978-3-642-37925-3_156, Ó Springer-Verlag Berlin Heidelberg 2014
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studies may be harmful to the commercial process of 2,3-BD product by microbial fermentation. As an abundant lignocellulosic by-product in the main agricultural countries, corncob has certain positive benefits such as renewability, large storage, environment-friendly, and low cost [9–11]. The unusually high content (35–40 %, w/w) of hemicelluloses in corncob justifies it as an attractive feedstock for pentose-based biotransformation aimed at production of xylitol, bulk chemicals [12], and bioethanol [13]. However, the waste residue rich in cellulose is discarded or furnace-burned in many cases, this is a great waste of natural resources, and this may damage the environment because of the chemical reagent which reacts with the corncob. Therefore, it is significant to utilize this valuable cellulosic residue in an economical and environmen
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