Corpora lutea affect in vitro maturation of bovine cumulus-oocyte complexes and embryonic development after fertilizatio
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Corpora lutea affect in vitro maturation of bovine cumulus-oocyte complexes and embryonic development after fertilization with sex-sorted or conventional semen Andrés Quezada-Casasola 1 & Haze Pamela Roldán-Domínguez 2 & Diego Eduardo Cano-Reagan 2 & Angélica María Escárcega-Ávila 1 & Mateo Fabian Itza-Ortiz 1 & José María Carrera-Chávez 1 & Ernesto Orozco-Lucero 1 Received: 28 February 2020 / Accepted: 10 September 2020 # Springer Nature B.V. 2020
Abstract Influence of both the presence of a corpus luteum on the ovary and semen sex-sorting on development following in vitro fertilization is not yet conclusive. To determine the effect of these factors, 376 bovine oocytes were processed in vitro according to luteal presence on the ovary (CL+ and CL−) and type of semen used (sexed or conventional). Maturation rate was higher (P < 0.01) in CL− (136/138; 98.6%) than in CL+ (217/238; 91.2%). Cleavage rate was lower (P < 0.01) in CL+ with sexed semen (60/172; 34.9%) than in CL− with sexed semen (42/71; 59.1%), CL+ with conventional semen (47/66; 71.2%), and CL− with conventional semen (54/67; 85.1%). Compaction was similar (P = 0.69) in CL− (49/99; 49.4%) and CL+ (50/107; 46.7%). Blastulation rate was higher (P < 0.01) in CL− (26/99, 26.2%) than in CL+ (13/107; 12.1%) group. Expansion rate was higher (P = 0.01) in CL− (22/99; 22%) than in CL+ (11/107; 10.2%) group. Compaction rates were similar (P = 0.78) in sex-sorted (50/ 102; 49.0%) or conventional semen (49/104; 47.1%) groups. Blastulation was also similar (P = 0.91) with sex-sorted semen (19/ 102; 18.6%) and conventional semen (20/104; 19.2%). The rate of expanded blastocysts was similar (P = 0.89) in sex-sorted (16/ 102; 15.6%) and conventional (17/104; 16.3%) semen groups. In conclusion, the presence of CL can compromise maturation of the oocytes and their development, as a higher proportion of cleavage-stage embryos can be obtained with non-sexed semen with oocytes from ovaries without a CL. Keywords Corpus luteum . Sexed semen . In vitro fertilization . Bovine oocytes
Introduction Embryo production by in vitro fertilization (IVF) of bovine embryos includes oocyte maturation, sperm capacitation followed by fertilization, and embryo culture. Oocyte maturation and sperm capacitation and fertilization are generally accomplished at approximately 24 and 48 h after oocyte collection, respectively, while embryo culture requires approximately 7 days. Oocytes can be obtained either by follicular aspiration in live cows and heifers or by using ovaries from abattoirs
* Ernesto Orozco-Lucero [email protected] 1
Departamento de Ciencias Veterinarias, Universidad Autónoma de Ciudad Juárez, Anillo envolvente Pronaf y Estocolmo, 32310 Ciudad Juárez, Chihuahua, Mexico
2
Lechería Escobar S.A. de C.V., Carretera Juárez-Porvenir Km 18.2., 32700 Ciudad Juárez, Chihuahua, Mexico
(Rehman et al., 2001). The early embryonic development includes cleavage, compaction, blastulation, expansion, hatching, and elongation. These events are essential for the successf
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