Cysteamine supplementation during in vitro maturation (IVM) of rabbit oocyte improves the developmental capacity after i
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ORIGINAL ARTICLE
Cysteamine supplementation during in vitro maturation (IVM) of rabbit oocyte improves the developmental capacity after intracytoplasmic sperm injection Hiroki Izumi • Yuki Miyamoto • Tatsufumi Mori • Yuka Hashigami • Yasutaka Chiba • Takeshi Teramura • Shu Hashimoto • Kanji Fukuda Yoshiharu Morimoto • Yoshihiko Hosoi
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Received: 6 November 2012 / Accepted: 4 June 2013 Ó Japan Society for Reproductive Medicine 2013
Abstract Purpose Current approaches to in vitro maturation (IVM) may result in low efficiency and inadequate quality of the oocytes due to insufficient cytoplasmic maturation. Although positive effects of the cysteamine supplementation in IVM medium for oocyte nuclear maturation or male pronuclear formation have been confirmed, it is still controversial whether the cysteamine addition affects embryo development after IVM. We aimed here to confirm the effect of cysteamine addition into IVM medium for subsequent embryo development in vitro. Methods We administered the cysteamine to the IVM culture of rabbit immature oocytes at various concentrations and observed the developmental rate, speed to reach blastocyst stage and cell numbers at the blastocyst stage. Results Cysteamine supplementation improved developmental rate to blastocyst stage of the IVM oocytes. On the other hand, addition of glutathione (GSH) inhibitor buthionine sulfoximine inhibited GSH accumulation in the
H. Izumi Y. Miyamoto T. Mori Y. Hashigami Y. Hosoi Department of Biology Oriented Science and Technology, Kinki University, Wakayama, Japan H. Izumi Y. Miyamoto S. Hashimoto Y. Morimoto IVF Namba Clinic, Osaka, Japan Y. Chiba Faculty of Medicine, Clinical Research Center, Kinki University, Osaka, Japan T. Teramura (&) K. Fukuda Faculty of Medicine, Institute of Advanced Clinical Medicine, Kinki University, 377-2 Ohno-higashi, Osaka-sayama, Osaka 5898511, Japan e-mail: [email protected]
oocytes and subsequent embryo development to the blastocyst stage. Conclusions Controlling the GSH quantity of IVM oocytes may be an important factor for success of embryo development, and it is quite probable that a cysteamine supplementation can contribute to an increase of GSH content in oocyte. Keywords Cysteamine Glutathione Intracytoplasmic sperm injection (ICSI) In vitro maturation (IVM) Rabbit
Introduction Important challenges in assisted reproductive technologies (ART) are reducing the amount of gonadotropins for oocyte collection in in vitro fertilization (IVF) to avoid the risk of ovarian hyperstimulation syndrome (OHSS), and enhancing the numbers and improving the qualities of the usable oocytes in poor responders to conventional gonadotropin therapies. For current practice in some IVF clinics, in vitro maturation (IVM) of oocytes, which can be collected from pre-matured antral follicles, is thought as possibly an effective procedure for the oocyte preparation in ART. IVM is a process by which immature oocytes are harvested from the ovaries and induced maturation and resumption of meiosis b
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