Effects of leptin on in vitro maturation, fertilization and embryonic cleavage after ICSI and early developmental expres
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BioMed Central
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Effects of leptin on in vitro maturation, fertilization and embryonic cleavage after ICSI and early developmental expression of leptin (Ob) and leptin receptor (ObR) proteins in the horse Anna Lange Consiglio1, Maria Elena Dell'Aquila2, Nadia Fiandanese2, Barbara Ambruosi2, Yoon S Cho4, Giampaolo Bosi3, Silvana Arrighi3, Giovanni M Lacalandra2 and Fausto Cremonesi*1 Address: 1Università degli Studi di Milano, Reproduction Unit, Large Animal Hospital, Faculty of Veterinary Medicine, Via dell'Università 6,-26900 Lodi, Italy, 2Department of Animal Production, Faculty of Biotechnological Sciences, S Prov Casamassima, km 3 - 70010 Valenzano (Bari), Italy, 3Department of Veterinary Science and Technologies for Food Safety, Laboratory of Anatomy, - via Trentacoste, 2 - 20134 Milano, Italy and 4Assisted Procreation Unit, Clinica Santa Maria, Bari, Italy Email: Anna Lange Consiglio - [email protected]; Maria Elena Dell'Aquila - [email protected]; Nadia Fiandanese - [email protected]; Barbara Ambruosi - [email protected]; Yoon S Cho - [email protected]; Giampaolo Bosi - [email protected]; Silvana Arrighi - [email protected]; Giovanni M Lacalandra - [email protected]; Fausto Cremonesi* - [email protected] * Corresponding author
Published: 16 October 2009 Reproductive Biology and Endocrinology 2009, 7:113
doi:10.1186/1477-7827-7-113
Received: 16 July 2009 Accepted: 16 October 2009
This article is available from: http://www.rbej.com/content/7/1/113 © 2009 Lange Consiglio et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract Background: The identification of the adipocyte-derived obesity gene product, leptin (Ob), and subsequently its association with reproduction in rodents and humans led to speculations that leptin may be involved in the regulation of oocyte and preimplantation embryo development. In mice and pigs, in vitro leptin addition significantly increased meiotic resumption and promoted preimplantation embryo development in a dose-dependent manner. This study was conducted to determine whether leptin supplementation during in vitro maturation (IVM) to horse oocytes could have effects on their developmental capacity after fertilization by IntraCytoplasmic Sperm Injection (ICSI). Methods: Compact and expanded-cumulus horse oocytes were matured in medium containing different concentrations (1, 10, 100, 1000 ng/ml) of recombinant human leptin and the effects on maturation, fertilization and embryo cleavage were evaluated. Furthermore, early developmental expression of Ob and leptin receptor (Ob-R) was investigated by immunocytochemical staining. Results: In expanded-cumulus oocytes, the addition of leptin in IVM medium improved mat
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