Correction to: A role for tetraspanin proteins in regulating fusion induced by Burkholderia thailandensis
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CORRECTION
Correction to: A role for tetraspanin proteins in regulating fusion induced by Burkholderia thailandensis Atiga Elgawidi1 · Muslim Idan Mohsin1,3 · Fawwaz Ali1,4 · Amyleigh Watts1 · Peter N. Monk2 · Mark S. Thomas2 · Lynda J. Partridge1
© Springer-Verlag GmbH Germany, part of Springer Nature 2020
Correction to: Medical Microbiology and Immunology https://doi.org/10.1007/s00430-020-00670-6
The original article has been corrected.
In the original article, incorrect Figures were published with incorrect captions. The correct Figs. 1, 2, 3, 4, 5, 6, 7 and 8 and captions are given below.
The original article can be found online at https://doi.org/10.1007/ s00430-020-00670-6. * Lynda J. Partridge [email protected] 1
Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield S10 2TN, UK
2
Department of Infection, Immunity and Cardiovascular Disease, Medical School, University of Sheffield, Sheffield S10 2RX, UK
3
Present Address: Department of Pathological Analyses, University of Kufa, Kufa, Iraq
4
Present Address: Mosul Technical Institute, Northern Technical University, Mosul, Iraq
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Vol.:(0123456789)
Medical Microbiology and Immunology
A 60
J774.2 CDC272 E264
MNGC size (nuclei/MNGC)
% MNGC formation
80
40 20 0
6hr
10hr
80 60
E264 WT
40 20 0
18hr
CDC272WT
6hr
Time
10hr
18hr
Time
RAW264.7
60
80
CDC272 E264
MNGC size (nuclei/MNGC)
% MNGC formation
80
40 20 0
8hr
12hr
24hr
13
E264
40 20 0
8hr
12hr
Time
Time
B
60
CDC272
a
b
c
d
24hr
Medical Microbiology and Immunology ◂Fig. 1 MNGC formation induced by B. thailandensis strains E264
and CDC272 in J774.2 and RAW264.7 mouse macrophages. A Time course of MNGC formation in J774.2 (upper panel) and RAW264.7 (lower panel) mouse macrophages induced by B. thailandensis strains E264 and CDC272. Cells were stained with Giemsa and the number of MNGC (cells with > 3 nuclei) was determined visually per field of view and expressed as a percentage of the total number of macrophages. The average size of MNGC was also calculated by determining the number of nuclei per MNGC per field of view. The error bars represent the standard error of the mean from three independent experiments, each comprising ten fields of view B. Representative images of Giemsa stained uninfected RAW264.7 cells (a) cells infected with B. thailandensis CDC272 (b) or E264 (c) for 16 h and with CDC272 for 24 h showing cell necrosis (d). Images were captured using by light microscopy using the 40 × objective, scale bar 20 μm
Expression [RFI]
25
cell surface expression
20
cytoplasmic expression
15 10 5
J774.2
81 D C
D 9 C D 63
C
C D 81
C
D
9 C D 63
0
RAW264.7
Fig. 2 Expression of CD9, CD63 and CD81 tetraspanins by J774.2 and RAW264.7 mouse macrophages. The relative levels of expression were measured by flow cytometry and are expressed as Relative Fluorescence Intensity (RFI) i.e. MFI of anti-tetraspanin antibody/ MFI isotype control. The data represent at least two experiments
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