Correction to: Proteotranscriptomics assisted gene annotation and spatial proteomics of Bombyx mori BmN4 cell line
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CORRECTION
Open Access
Correction to: Proteotranscriptomics assisted gene annotation and spatial proteomics of Bombyx mori BmN4 cell line Michal Levin*, Marion Scheibe and Falk Butter* Correction to: BMC Genomics 21, 690 (2020) https://doi.org/10.1186/s12864-020-07088-7 Following publication of the original article [1], it was reported that there were errors in Figs. 1 and 2. The correct figures are included in this Correction article and the original article has been updated.
Reference 1. Levin M, Scheibe M, Butter F. Proteotranscriptomics assisted gene annotation and spatial proteomics of Bombyx mori BmN4 cell line. BMC Genomics. 2020;21:690 https://doi.org/10.1186/s12864-020-07088-7.
The original article can be found online at https://doi.org/10.1186/s12864020-07088-7. * Correspondence: [email protected]; [email protected] Institute of Molecular Biology (IMB), Ackermannweg 4, 55128 Mainz, Germany © The Author(s). 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
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Fig. 1 Genome-free transcriptome assembly approach and assessment of annotation quality. a. Overview of the proteotranscriptomics annotation approach. b. Pie-chart of BUSCO analysis based on the BUSCO arthropoda gene set. c. Barplot summarizing the results of a full-length transcript comparison between the genome-free Trinity assembly to currently available annotations from UniProt, NCBI, SilkBase and SilkDB 3.0
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Fig. 2 High resolution mass spectrometry provides evidence for superior genome-free annotation. a. Violin plots show distribution of identified MS/MS spectra (in percent) for each database used. With identical raw proteomic data the genome-free Trinity annotation shows significantly higher identified tandem MS spectra percentages than the four currently available annotations from UniProt, NCBI, SilkBase and SilkDB 3.0. Grey lines connect percentages stemming from the same MS run. **** indicates two-sided paired Wilcoxon signed rank test p
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