Correlation between DNA methylation and Thymic Stromal Lymphopoietin expression in asthmatic airway epithelial cells
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Genes & Genomics https://doi.org/10.1007/s13258-020-01000-z
RESEARCH ARTICLE
Correlation between DNA methylation and Thymic Stromal Lymphopoietin expression in asthmatic airway epithelial cells Yan‑Li Li1,2 · Xi‑Qian Xing2 · Yi Xiao2 · Yan‑Hong Liu2 · Yu‑Shan Zhou2 · Min Zhuang2 · Chao‑Qian Li1 Received: 13 May 2020 / Accepted: 15 September 2020 © The Genetics Society of Korea 2020
Abstract Background The overexpression of TSLP and DNA methylation in asthma were both risk factors the relationship was not clear. Objective This study aimed to investigate the relationship between methylation status of TSLP promoter and mRNA/protein expression in asthmatic airway epithelial cells. Methods Human bronchial epithelial cells were cultured in vitro and divided into: Control group, treated with PBS, model group, sensitized with LPS (10 μg/mL) for 12 h (37 °C, 5% CO2). Other groups were cultured with the pCMV3 plasmid (M + NC/pCMV), pGPH1 plasmid (M + NC/pGPH), DNMT1/pCMV3 plasmid (M + DNMT1/pCMV), and DNMT1/pGPH1 plasmid (M + DNMT1/pGPH) for 48 h. The expression of DNA methyltransferase 1 and TSLP were measured using realtime PCR and western blotting. Results Compared with the control group, TSLP mRNA (1.00 ± 0.00 vs. 2.82 ± 0.81 vs. 1, P
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