Correlation between sestrin2 expression and airway remodeling in COPD
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RESEARCH ARTICLE
Open Access
Correlation between sestrin2 expression and airway remodeling in COPD Da-Wei Zhang1,2†, Yuan-Yuan Wei1,2†, Shuang Ji1,2 and Guang-He Fei1,2*
Abstract Background: Airway remodeling is a major pathological characteristic of chronic obstructive pulmonary disease (COPD), and has been shown to be associated with oxidative stress. Sestrin2 has recently drawn attention as an important antioxidant protein. However, the underlying correlation between sestrin2 and airway remodeling in COPD has yet to be clarified. Methods: A total of 124 subjects were enrolled in this study, including 62 control subjects and 62 COPD patients. The pathological changes in airway tissues were assessed by different staining methods. The expression of sestrin2 and matrix metalloproteinase 9 (MMP9) in airway tissues was monitored by immunohistochemistry. Enzyme-linked immunosorbent assays (ELISAs) were used to detect the serum concentrations of sestrin2 and MMP9. The airway parameters on computed tomography (CT) from all participants were measured for evaluating airway remodeling. The relationship between serum sestrin2 and MMP9 concentration and airway parameters in chest CT was also analyzed. Results: In patients with COPD, staining of airway structures showed distinct pathological changes of remodeling, including cilia cluttered, subepithelial fibrosis, and reticular basement membrane (Rbm) fragmentation. Compared with control subjects, the expression of sestrin2 and MMP9 was significantly increased in both human airway tissues and serum. Typical imaging characteristics of airway remodeling and increased airway parameters were also found by chest CT. Additionally, serum sestrin2 concentration was positively correlated with serum MMP9 concentration and airway parameters in chest CT. Conclusion: Increased expression of sestrin2 is related to airway remodeling in COPD. We demonstrated for the first time that sestrin2 may be a novel biomarker for airway remodeling in patients with COPD. Keywords: Sestrin2, Airway remodeling, COPD, Matrix metalloproteinase 9, Quantitative computed tomography
Background Chronic obstructive pulmonary disease (COPD) is the most common chronic airway inflammatory disease, and is characterized by persistent respiratory symptoms and irreversible airflow limitation. The pathological changes that contribute to the loss of lung function in COPD * Correspondence: [email protected] † Da-Wei Zhang and Yuan-Yuan Wei contributed equally to this work. 1 Department of Respiratory and Critical Care Medicine, First Affiliated Hospital of Anhui Medical University, Hefei 230022, Anhui Province, People’s Republic of China 2 Key Laboratory of Respiratory Diseases Research and Medical Transformation of Anhui Province, Hefei 230022, Anhui Province, People’s Republic of China
consist of parenchymal destruction (described clinically as emphysema), remodeling of the airways (described clinically as small airways disease), and mucus hypersecretion (described clinically as chronic bronchitis [1] . Airway remod
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