Cryopreservation of Mammalian Gametes and Embryos Methods and Protoc
This volume provides a comprehensive and technical presentation of numerous aspects of reproductive cell tissue cryopreservation, and presents readers with current procedures and detailed discussions of novel techniques and the latest innovations. The cha
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Zsolt Peter Nagy Alex C. Varghese Ashok Agarwal Editors
Cryopreservation of Mammalian Gametes and Embryos Methods and Protocols
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Cryopreservation of Mammalian Gametes and Embryos Methods and Protocols
Edited by
Zsolt Peter Nagy Reproductive Biology Associates Atlanta, GA, USA
Alex C. Varghese ASTRA Fertility Group Mississauga, ON, Canada
Ashok Agarwal Cleveland Clinic Foundation Cleveland, OH, USA
Editors Zsolt Peter Nagy Reproductive Biology Associates Atlanta, GA, USA
Alex C. Varghese ASTRA Fertility Group Mississauga, ON, Canada
Ashok Agarwal Cleveland Clinic Foundation Cleveland, OH, USA
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-6826-8 ISBN 978-1-4939-6828-2 (eBook) DOI 10.1007/978-1-4939-6828-2 Library of Congress Control Number: 2017934237 © Springer Science+Business Media LLC 2017 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Printed on acid-free paper This Humana Press imprint is published by Springer Nature The registered company is Springer Science+Business Media LLC The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.
Preface Cryopreservation of oocytes, sperm, and ovarian and testicular tissues, as well as embryos, is one of the most critical procedures to preserve the reproductive capacity of individuals. To grasp its importance it is essential to know that in the United States alone, there are over forty thousand women younger than 40 years old who develop malignant tumors every year—many of whom do not have children/families and whose fertility capacity is at risk because of the disease or the medical therapy applied against the disease. T
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