Cryopreservation of mouse resources
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Laboratory Animal Research
REVIEW
Open Access
Cryopreservation of mouse resources Toru Takeo1* , Satohiro Nakao1, Yoshiko Nakagawa1, Jorge M. Sztein1 and Naomi Nakagata2
Abstract The cryopreservation of sperm and embryos is useful to efficiently archive valuable resources of genetically engineered mice. Till date, more than 60,000 strains of genetically engineered mice have been archived in mouse banks worldwide. Researchers can request for the archived mouse strains for their research projects. The research infrastructure of mouse banks improves the availability of mouse resources, the productivity of research projects, and the reproducibility of animal experiments. Our research team manages the mouse bank at the Center for Animal Resources and Development in Kumamoto University and continuously develops new techniques in mouse reproductive technology to efficiently improve the system of mouse banking. In this review, we introduce the activities of mouse banks and the latest techniques used in mouse reproductive technology. Keywords: Genetically engineered mice, Mouse bank, Reproductive technology, Sperm, Embryo, Cryopreservation, In vitro fertilization, Cold storage, Hands-on workshop
Introduction A genetically engineered mouse is a powerful tool to elucidate the complex communications between genes or organs in health and diseases [1]. Moreover, humanized mouse models derived from immunosuppressed mice are helpful to bridge the gap of discovery and the development of new medicines between human and animal experiments [2]. Therefore, it is important to enhance the availability and accessibility of mouse resources to conduct research projects efficiently using valuable mouse models. A mouse bank plays a vital role in archiving and supplying mouse resources [3]. In Kumamoto University, the Center for Animal Resources and Development (CARD) was established as a research center for genetics and biomedical science using genetically engineered mice and as a quality center of mouse resources as a mouse bank in 1998 [4, 5]. The CARD provides services of production, cryopreservation, and supply of genetically engineered mice and established a searchable database of the archived mouse strains known as the CARD * Correspondence: [email protected] 1 Division of Reproductive Engineering, Center for Animal Resources and Development, Institute of Resource Development and Analysis, Kumamoto University, 2-2-1 Honjo, Kumamoto 860-0811, Japan Full list of author information is available at the end of the article
Resource Database (CARD R-BASE, http://cardb.cc.kumamoto-u.ac.jp/transgenic/). Till date, the international collaboration of mouse banks (International Mouse Strain Resource: IMSR) has successfully collected more than 60,000 strains of genetically engineered mice (Table 1). The archived mouse strains can be browsed through the IMSR website (http://www.findmice.org/) [6]. Researchers can obtain live mice, cryopreserved embryos, or the sperm of choice from those mouse banks. In Asia, an international association of
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