Dan Purich (Author): Enzyme Kinetics: Catalysis & Control: A Reference of Theory and Best-Practice Methods
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BOOK REVIEW
Dan Purich (Author): Enzyme Kinetics: Catalysis & Control: A Reference of Theory and Best-Practice Methods Elsevier, Copyright: 2010. Format: Hardback. Extent: 920. Price: $210. ISBN: 978-0-12-380924-7 P. A. Tipton
Received: 8 March 2012 / Accepted: 20 April 2012 / Published online: 8 May 2012 Ó Springer Science+Business Media, LLC 2012
There is a danger of shock upon picking up Enzyme Kinetics; Catalysis and Control and realizing that it is almost 900 pages long. How much enzyme kinetics could there possibly be, or, more to the point, how much enzyme kinetics does one really need? The prospect of leafing through page after page of algebraic equations and double reciprocal plots can cause sweaty palms in even the most hardened enzymologist. Panic wanes in the reader of Enzyme Kinetics, however, when it becomes apparent that kinetics is only a portion of the material that is presented. In fact, reading the book is downright enjoyable because the author, Daniel Purich, has written an engaging, wideranging book that covers many topics beyond the purview of kinetics as it is usually defined. If the title Enzyme Kinetics is something of a misnomer, the sub-title, A Reference of Theory and Best Practice Methods is not. This book is not designed to be used as a textbook and the broad range of topics that are covered warrants the classification as a reference. The level at which chemical concepts are presented is appropriate for advanced students in biochemistry or chemistry; mathematical explanations sometimes rely on linear algebra and calculus. In addition to detailed sections covering standard topics in steady-state kinetics such as derivation of rate equations and kinetics of inhibitors and multisubstrate reactions, the book includes sections on practical topics such as how to design an assay, how to design and interpret site-directed mutagenesis experiments, and how to apply different isotope exchange techniques. A welcome component of the book is a discussion of the different software P. A. Tipton (&) Department of Biochemistry, University of Missouri, Columbia, MO, USA e-mail: [email protected]
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packages that are available for data analysis, including global fitting software packages used for transient-state kinetic data. In contrast to many books devoted to enzyme kinetics, Purich devotes considerable space to discussions of the basic chemistry that underlies many enzymatic reactions or the techniques used to monitor them. Thus, one finds a brief introduction to coordination chemistry and to the different classes of covalent bond-making and -breaking reactions in the chapter entitled ‘‘Active Sites and their Chemical Properties’’. Discussions of kinetic experiments treat both introductory considerations (for fluorescence measurements: ‘‘Step 1. Place a fluorescence cuvette containing an adequate volume of sample within the instrument’’.) and more sophisticated topics such as the proper statistical analysis of kinetic data, and the different methods used in single-molecule kinetic studies. Ther
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