Enzyme Stabilization and Immobilization Methods and Protocols
This volume introduces the reader to the field of enzyme stabilization and the different theories of enzyme stabilization, including the use of immobilization as a stabilization technique. The first part of the book focuses on protocols for enzyme stabili
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Shelley D. Minteer Editor
Enzyme Stabilization and Immobilization Methods and Protocols Second Edition
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Enzyme Stabilization and Immobilization Methods and Protocols Second Edition
Edited by
Shelley D. Minteer Departments of Chemistry and Department of Materials Science and Engineering, University of Utah, Salt Lake City, UT, USA
Editor Shelley D. Minteer Departments of Chemistry and Materials Science and Engineering University of Utah Salt Lake City, UT, USA
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-6497-0 ISBN 978-1-4939-6499-4 (eBook) DOI 10.1007/978-1-4939-6499-4 Library of Congress Control Number: 2016951931 © Springer Science+Business Media New York 2017 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. Printed on acid-free paper This Humana Press imprint is published by Springer Nature The registered company is Springer Science+Business Media LLC New York
Preface Enzyme stabilization has been an area of research interest since the 1950s, but in the last three decades, researchers have made tremendous progress in the field. This has opened up new opportunities for enzymes in molecular biology as well as industrial applications, such as bioprocessing, biosensors, and biofuel cells. The first chapter introduces the reader to the field of enzyme stabilization and the different theories of enzyme stabilization, including the use of immobilization as a stabilization technique. The first part of the book will focus on protocols for enzyme stabilization in solutions including liposome formation, micelle introduction, crosslinking, and additives, while the second part of the book will focus on protocols for enzyme stabilization during enzyme immobilization including common techniques like sol-gel encapsula
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