Design of a DNA-Based Biomaterial by Sol-Gel Method: Application for the Recognition of Albendazole Sulfoxide
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MRS Advances © 2019 Materials Research Society DOI: 10.1557/adv.2019.417
Design of a DNA-Based Biomaterial by Sol-Gel Method: Application for the Recognition of Albendazole Sulfoxide Elani J. Cabrera-Vega1, Georgina Alarcón-Ángeles1, Martín Gómez Hernández1, Gonzalo Campillo-Alvarado2, Marcela Hurtado Y De la Peña1,* 1
Departamento de Sistemas Biológicos, Universidad Autónoma Metropolitana Xochimilco, México, D.F. 04960, México. 2Department of Chemistry, University of Iowa, Iowa City, Iowa 52242, United States.
ABSTRACT
We describe the use of immobilized deoxyribonucleic acid (DNA) in a silica matrix as a biorecognition agent for the detection of albendazole sulfoxide (ASU), the primary metabolite of albendazole and a suspected teratogenic and embryotoxic agent. The biomaterial (DNAcontaining gel) was synthesized by physical entrapment of salmon sperm in an inorganic silicate matrix by the sol-gel method. Functionality of the DNA-containing gel was evaluated by comparative offline frontal chromatography followed by HPLC analysis of ASU and caffeine (CAF, control) using DNA-containing gel and DNA-free gel. The DNA-containing gel showed relatively high specific retention for ASU, while CAF showed no retention using frontal analysis. We anticipate that the DNA-containing gel can be implemented to identify the interactions of DNA with other active pharmaceutical ingredients (APIs) and their metabolites in a readily available, sensitive and selective frontal chromatography experiment.
INTRODUCTION Deoxyribonucleic acid (DNA) is a natural molecule that has emerged as a versatile building block in the field of materials. [1,2] The remarkable binding specificity and thermodynamic properties derived from the robust and programmable structural features (e.g., electrostatic forces, the matching of complementary bases and intercalation and union between the grooves [3]) make DNA an ideal molecule to confer function. For instance, biomaterials with immobilized DNA have been explored as adsorbants for environmental clean-up, thus constituting a versatile analytical tool for the recognition of metal complexes, polycyclic aromatic hydrocarbons (PHAs), and endocrine disruptors found in water. Likewise, DNA immobilization has been used for the design of
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chromatographic stationary phases for the identification of anticarcinogenic components in herbalist products. [4-10] However, to the best of our knowledge, DNA-containing biomaterials have not been previously implemented in the context of recognition of active pharmaceutical ingredients (APIs) and their metabolites. The design of a DNAcontaining biomaterial would thus constitute a promising in vitro tool for the identification of APIs with therapeutic or genotoxic effects having DNA as the primary biological target. [11] Immobilization usin
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