Design strategy and bioimaging of small organic molecule multicolor fluorescent probes
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sign strategy and bioimaging of small organic molecule multicolor fluorescent probes 1
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Yongfei Huang , Yongbin Zhang , Fangjun Huo , Ying Wen & Caixia Yin 1
Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Molecular Science, Shanxi University, Taiyuan 030006, China; Key Laboratory of Functional Molecules of Shanxi Province, Research Institute of Applied Chemistry, Shanxi University,
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Taiyuan 030006, China Received July 13, 2020; accepted August 25, 2020; published online October 21, 2020
Multicolor fluorescent probes based on small organic molecules have the advantages of low cost, good biocompatibility, easily modifiable molecular structures and adjustable fluorescence performance. In addition, small molecule multicolor fluorescent probes generally undergo multi-site or multi-step reactions, which means that they can be used for the specific detection of structurally similar substances in complex bio-systems. In this review, we focus on the design and application of multicolor fluorescent probes based on small organic molecules: single fluorophores with multiple reaction sites, multiple fluorophores with single reaction sites, or multiple fluorophores with multiple reaction sites. Moreover, a design strategy for multicolor fluorescent probes and its application in biological imaging are also summarized, providing a systematic plan for future research on fluorescent probes functionalized by small organic molecules. It will also play an important role in the development of additional functions for small organic molecule fluorescent probes. multicolor, fluorescence probes, small organic molecules, design strategy, bioimaging Citation:
Huang Y, Zhang Y, Huo F, Wen Y, Yin C. Design strategy and bioimaging of small organic molecule multicolor fluorescent probes. Sci China Chem, 2020, 63, https://doi.org/10.1007/s11426-020-9855-3
1 Introduction Fluorescence detection is a powerful tool for labelling and investigating the sensing of bio-analytes [1]. Fluorescent probes based on small organic molecules have become more and more popular due to their unique advantages, such as their easily modifiable molecular structures, adjustable fluorescence performance, relatively quick responses and good biocompatibility [2–5]. Many excellent probes have been developed for the detection of disease related substances, including thiols, metal ions, reactive oxygen species (ROS) and reactive sulfide species (RSS), among others, and many can detect aspects of the intracellular environment *Corresponding author (email: [email protected])
(such as pH, polarity and viscosity) [6–25]. To date, some important review articles have been published, summarizing notable work on fluorescent probes [26–32]. A new trend over the past five years has been the synthesis of stimuli-responsive multicolor emissions materials through different chemical design strategies. Multicolor emissions materials have diverse applications in optical recording, optical sensors, switches, molecular machi
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