Development and characterization of microsatellite markers in Stryphnodendron adstringens (Leguminosae)
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Development and characterization of microsatellite markers in Stryphnodendron adstringens (Leguminosae) Ariany Rosa Gonc¸alves1,2 • Luciana Oliveira Barateli1,2 • Ueric Jose´ Borges de Souza1,2 • Ana Maria Soares Pereira3 • Bianca Wale´ria Bertoni3 • Mariana Pires de Campos Telles2,4
Received: 4 March 2020 / Revised: 3 August 2020 / Accepted: 26 August 2020 Ó Prof. H.S. Srivastava Foundation for Science and Society 2020
Abstract In this study, we report the development and characterization of 15 new microsatellite markers for Stryphnodendron adstringens (Leguminosae) in order to support future analyses of genetic diversity in populations of this species. In screening with 48 individuals from three different populations of S. adstringens, we tested the amplification of 20 microsatellite loci, of which five are not useful for population genetic studies due to the lack of polymorphisms or amplification failures. For the final set of 15 loci, the number of alleles ranged from 2 to 15, with a total of 116 alleles. The expected heterozygosity ranged from 0.1219 to 0.8965, with an average of 0.6694 per locus. The combined probability of genetic identity (PI = 8.12 9 10-15) and paternity exclusion (Q = 0.99999) estimations showed that the loci may be useful to discriminate between individuals of S. adstringens. Initial cross-amplification tests were satisfactory in three species of the genus Stryphnodendron: S.
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s12298-020-00876-1) contains supplementary material, which is available to authorized users. & Mariana Pires de Campos Telles [email protected] 1
Programa de Po´s-graduac¸a˜o em Gene´tica e Melhoramento de Plantas, Escola de Agronomia, Universidade Federal de Goia´s, Goiaˆnia, Goia´s, Brazil
2
Laborato´rio de Gene´tica e Biodiversidade, Instituto de Cieˆncias Biolo´gicas, Universidade Federal de Goia´s, Goiaˆnia, Goia´s, Brazil
3
Unidade de Biotecnologia, Universidade de Ribeira˜o Preto, Ribeira˜o Preto, Sa˜o Paulo, Brazil
4
Escola de Cieˆncias Agra´rias e Biolo´gicas, Pontifı´cia Universidade Cato´lica de Goia´s, Goiaˆnia, Goia´s, Brazil
rotundifolium, S. coriaceum and S. polyphyllum. This new set of markers will be a useful tool for population genetic studies, contributing to the knowledge about the evolutionary history of S. adstringens and, additionally, correlated species. Keywords Cerrado Genetic diversity Next Generation Sequencing Medicinal plant SSR markers
Introduction The genus Stryphnodendron Mart. (Leguminosae family) has actually 25 recognized species, of which 21 occur in Brazil (Souza and Lima 2020). Among several plant species native to Brazil, Stryphnodendron adstringens (Mart.) Coville stands out because of its medicinal relevance, whose antioxidant, antimicrobial and antitumor properties have already been highlighted in several clinical studies (see Souza-Moreira et al. 2018; Pellenz et al. 2019). This species, popularly known as barbatima˜o, is widely distribut
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