Development and characterization of nine polymorphic microsatellite markers for the blue sheep ( Pseudois nayaur )
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MICROSATELLITE LETTERS
Development and characterization of nine polymorphic microsatellite markers for the blue sheep (Pseudois nayaur) Min Yang • Quekun Peng • Lichun Jiang Shuai Tan • Rui Peng • Fangdong Zou
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Received: 13 August 2014 / Accepted: 28 August 2014 Ó Springer Science+Business Media Dordrecht 2014
Abstract Pseudois nayaur is a major component of Caprini and a keystone species in Qinghai-Tibet Plateau ecosystems. Due to the excessive hunting and habitat loss, its numbers are being reduced drastically. We developed and characterized the first set of microsatellite markers in this species to investigate its population genetic diversity and structure. Nine polymorphic loci displayed an average of 5.73 alleles (range between 2 and 14) and the levels of expected heterozygosity ranged from 0.422 to 0.816. Cross-amplification within dwarf blue sheep was successful for the nine loci. The loci characterized here will be useful for detecting fine-scale spatial structuring and resolving the taxonomic status of divergent Pseudois populations. Keywords Pseudois nayaur Microsatellite markers Polymorphism Population genetics
Min Yang and Quekun Peng have contributed equally to this work. M. Yang L. Jiang S. Tan R. Peng F. Zou (&) Sichuan Key Laboratory of Conservation Biology on Endangered Wildlife, Key Laboratory of Bio-resources and Ecoenvironment (Ministry of Education), College of Life Sciences, Sichuan University, Chengdu 610065, Sichuan, People’s Republic of China e-mail: [email protected] Q. Peng Department of Biomedical Science, Chengdu Medical College, Tian Hui Road 601, Chengdu 610083, Sichuan, People’s Republic of China
Blue sheep (Pseudois nayaur), a Central Asian ungulate with restricted geographic distribution, exhibits extensive variation in morphology and phylogeographic structure (Zeng et al. 2008). The composition of species and subspecies in the genus Pseudois is controversial, particularly with respect to the taxonomic designation of geographically restricted populations (Tan et al. 2012). The genus Pseudois includes two morphologically variable taxa, blue sheep (P. nayaur) and dwarf blue sheep (P. schaeferi). Blue sheep have a wide distribution range across the entire Tibetan Plateau region, while dwarf blue sheep were only found in the gorges of the upper Yangtze River (Jinsha River) near Batang county, Sichuan province. As the body size of dwarf blue sheep is only about a half of blue sheep, its taxonomic status in the genus Pseudois has been argued for several decades since its discovery in 1937 (Schafer 1937). More genetic evidences from blue sheep and dwarf blue sheep are required to clarify this question. The (AC)n microsatellite-enriched libraries were constructed based on the protocol described by Peng et al. (2011). The primers amplifying the microsatellites were designed using the software PRIMER5. Finally, 9 primer pairs were characterized using PCRs (Table 1). The primers that were characterized successfully using PCR were labeled with fluorescence and PCR produ
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