Development and characterization of microsatellite markers for the finetooth shark, Carcharhinus isodon
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TECHNICAL NOTE
Development and characterization of microsatellite markers for the finetooth shark, Carcharhinus isodon Melissa Giresi • Mark A. Renshaw David S. Portnoy • John R. Gold
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Received: 20 January 2012 / Accepted: 8 February 2012 / Published online: 22 February 2012 Ó Springer Science+Business Media B.V. 2012
Abstract The finetooth shark, Carcharhinus isodon, is a small, discontinuously distributed coastal species found in waters of the western Atlantic Ocean from North Carolina in the US through the Gulf of Mexico and in southern Brazil. The species is a component of both commercial and recreational fisheries throughout its range. Here, we report polymerase-chain-reaction primers for 20 polymorphic and 33 monomorphic microsatellites isolated from an enriched genomic library of C. isodon DNA, and cross amplification of nine polymorphic and 35 monomorphic microsatellites previously characterized in blacknose shark, C. acronotus. All 97 microsatellites were characterized in 31 individuals. The microsatellites will be useful in population studies of finetooth sharks. Keywords Microsatellites Finetooth shark Carcharhinus isodon Cross-amplification
The finetooth shark, Carcharhinus isodon, is a small, migratory coastal species with a discontinuous distribution in the western Atlantic Ocean. It has been reported to occur along the continental shelf from North Carolina in the United States (US) to Mexico, in Cuba, and in southern Brazil (Compagno et al. 2005). Although data on population structure of C. isodon are limited, differences in lifehistory characters between finetooth sharks along the eastern coast of the US and those in the Gulf of Mexico are compatible with the hypothesis that there may be separate reproductive units in US waters (Carlson et al. 2003; M. Giresi (&) M. A. Renshaw D. S. Portnoy J. R. Gold Center for Biosystematics and Biodiversity, Texas A and M University, College Station, TX 77843-2258, USA e-mail: [email protected]
Drymon et al. 2006; Driggers and Hoffmayer 2009). A recent stock assessment (NMFS 2007) indicated that finetooth sharks in US waters are not currently overfished or experiencing overfishing; however, it was noted that there is less catch and life-history data available for finetooth sharks than for other, small coastal species and that managers should proceed cautiously (NMFS 2007). Here, we describe development and characterization of 52 microsatellites from an enriched genomic library of C. isodon DNA and cross-amplification and characterization of an additional 44 microsatellites developed for the blacknose shark, C. acronotus (Giresi et al. 2011). The microsatellites will be useful in assessing stock structure of finetooth sharks and thus contribute to better-informed management of the species. Generation of an enriched genomic library of C. isodon DNA followed procedures outlined in Renshaw et al. (2010). A hybridization reaction was performed using 50pmol of a 30 -biotin modified (CA)13 oligonucleotide. The hybridization mixture was heated to 95°C for
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