Development of 15 polymorphic microsatellite markers for the shortfin mako, Isurus oxyrinchus , and cross-species amplif
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TECHNICAL NOTE
Development of 15 polymorphic microsatellite markers for the shortfin mako, Isurus oxyrinchus, and cross-species amplification in lamniforme sharks Mioko Taguchi • Toru Kitamura • Yuya Shigenobu • Makoto Ohkubo • Takashi Yanagimoto • Takuma Sugaya Yoji Nakamura • Kenji Saitoh • Kotaro Yokawa
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Received: 13 February 2013 / Accepted: 15 February 2013 / Published online: 23 February 2013 Ó Springer Science+Business Media Dordrecht 2013
Abstract The shortfin mako (Isurus oxyrinchus, Lamniformes) is one of common sharks caught incidentally by tuna longline and drift net fisheries in the tropical and temperate waters. However, their genetic diversity and population structure for effective management remain to be elucidated with sufficient number of genetic markers. A total of fifteen novel microsatellite markers for the shortfin mako were developed using a next generation sequencing approach. All the microsatellite loci isolated were polymorphic with 2–23 alleles, with the observed and expected heterozygosities of 0.06–1.00 and 0.06–0.93, respectively. Cross-species amplification in six other lamniforme sharks
M. Taguchi (&) K. Yokawa National Research Institute of Far Seas Fisheries, 5-7-1 Orido, Shimizu-ku, Shizuoka 424-8633, Japan e-mail: [email protected] T. Kitamura JAPAN NUS CO., LTD., Nishi-Shinjuku Kimuraya Bldg., 5F, 7-5-25 Nishi-Shinjuku, Shinjuku-ku, Tokyo 160-0023, Japan Y. Shigenobu M. Ohkubo T. Yanagimoto T. Sugaya Y. Nakamura K. Saitoh National Research Institute of Fisheries Science, 2-12-4 Fukuura, Kanazawa, Yokohama, Kanagawa 236-8648, Japan Present Address: M. Ohkubo Department of Biology, Faculty of Education and Integrated Arts and Sciences, Waseda University, 2-2 Wakamatsu-cho, Shinjuku-ku, Tokyo 162-8480, Japan Present Address: T. Sugaya Momoshima Branch, National Research Institute of Fisheries and Environment of Inland Sea, 1760 Momoshima, Onomichi, Hiroshima 722-0061, Japan
was successful at thirteen out of fifteen loci isolated. The developed microsatellite markers will be useful for the population genetic analysis of lamniforme sharks including the shortfin mako. Keywords DNA marker Elasmobranchii Genetic structure Genetic diversity Shark Fisheries management
The shortfin mako (Isurus oxyrinchus) is a large cosmopolitan shark found in the tropical and temperate waters and often caught incidentally by tuna longline and drift net fisheries throughout the species’ range (e.g. Compagno 2001). Information on the genetic diversity and population structure of fisheries resources should be helpful to identify their management unit. Nevertheless, such population genetic studies are scarce in the shortfin mako (Heist et al. 1996; Schrey and Heist 2003), owing to insufficient number of available molecular genetic markers, such as microsatellite loci (Schrey and Heist 2002; Agostini et al. 2011). Here we isolated novel fifteen microsatellite loci for the shortfin mako using next generation sequencing approach, and conducted cross-species amplification to test
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