Development of novel microsatellite markers for Cymodocea rotundata Ehrenberg ( Cymodoceaceae ), a pioneer seagrass spec
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Development of novel microsatellite markers for Cymodocea rotundata Ehrenberg (Cymodoceaceae), a pioneer seagrass species widely distributed in the Indo-Pacific Dan M. Arriesgado • Yuichi Nakajima • Yu Matsuki • Chunlan Lian • Satoshi Nagai • Motoshige Yasuike • Yoji Nakamura • Miguel D. Fortes • Wilfredo H. Uy • Wilfredo L. Campos • Masahiro Nakaoka • Kazuo Nadaoka
Received: 22 July 2013 / Accepted: 12 August 2013 / Published online: 21 August 2013 Ó Springer Science+Business Media Dordrecht 2013
Abstract Genetic diversity and population genetic structure are key components of seagrass resilience and contribute to an understanding of its conservation and management. We isolated 29 polymorphic microsatellite (SSR) markers from a widely distributed pioneer seagrass, Cymodocea rotundata, by two methods; next generation sequencing and compound SSR marker isolation. Twentynine markers had 2–14 alleles per locus, and the observed and expected heterozygosity ranged from 0 to 0.688, and 0.113 to 0.770, respectively. These loci will facilitate investigation of the genetic diversity and population genetic connectivity and structure of C. rotundata. Keywords Clonal plant Simple sequence repeat Cymodocea rotundata Pioneer Seagrass
D. M. Arriesgado K. Nadaoka Department of Mechanical and Environmental Informatics, Graduate School of Information Science and Engineering, Tokyo Institute of Technology, 2-12-1 O-okayama, Meguro, Tokyo 152-8552, Japan D. M. Arriesgado Y. Nakajima Y. Matsuki C. Lian (&) Asian Natural Environmental Science Center, The University of Tokyo, 1-1-8 Midori-cho, Nishitokyo, Tokyo 188-0002, Japan e-mail: [email protected] D. M. Arriesgado W. H. Uy Institute of Fisheries Research and Development, Mindanao State University, 9023 Naawan, Misamis Oriental, Philippines
The pioneer species, Cymodocea rotundata, is one of the most important and widespread seagrasses. It can colonize diverse environments, and often forms a fringing bed, being present in areas exposed to impact from coastal development and high anthropogenic activity (Terrados et al. 1998). C. rotundata is a clonal plant that can reproduce either sexually by seed production or asexually by vegetative propagation of rhizomes. This species is included in the Catalogue of Endangered Species, yet no specific conservation measures are currently in place, and the genetics of this species have not been investigated. In this study, microsatellite (SSR) markers of C. rotundata were developed in order to assess the genetic diversity, connectivity and population genetic structure throughout its distribution range. Genomic DNA was extracted from silica-gel dried leaves of C. rotundata collected from Rizal, Zamboanga
M. D. Fortes Marine Science Institute CS, University of the Philippines, Diliman, 1101 Quezon City, Philippines W. L. Campos Division of Biological Sciences, College of Arts & Sciences, University of the Philippines Visayas, 5023 Miag-ao, Iloilo, Philippines M. Nakaoka Akkeshi Marine Station, Field
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