Development and characterization of twenty-two novel microsatellite markers for the mountain whitefish, Prosopium willia
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TECHNICAL NOTE
Development and characterization of twenty-two novel microsatellite markers for the mountain whitefish, Prosopium williamsoni and cross-amplification in the round whitefish, P. cylindraceum, using paired-end Illumina shotgun sequencing Jason O’Bryhim • Christopher Somers • Stacey L. Lance • Monica Yau • Douglas R. Boreham Kenneth L. Jones • Eric B. Taylor
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Received: 28 July 2012 / Accepted: 6 August 2012 / Published online: 14 August 2012 Ó Springer Science+Business Media B.V. 2012
Abstract We isolated and characterized a total of 22 microsatellite loci in Prosopium williamsoni. Loci were screened in 24 individuals from across a portion of its range in the Peace River area of northeastern British Columbia. The number of alleles per locus ranged from 3 to 19, observed heterozygosity ranged from 0.111 to 0.950, and the probability of identity values ranged from 0.013 to 0.604. These new loci will be used for conducting investigations into the genetic structure and diversity of extant populations of this important forage fish. The loci were also screened in the congener P. cylindraceum, which is of interest as an indicator species in the Great Lakes region of
J. O’Bryhim S. L. Lance Savannah River Ecology Laboratory, University of Georgia, Aiken, SC 29802, USA C. Somers Department of Biology, University of Regina, Regina, SK S4S 0A2, Canada M. Yau E. B. Taylor (&) Department of Zoology, Biodiversity Research Centre and Beaty Biodiversity Museum, University of British Columbia, Vancouver, BC V6T 1Z4, Canada e-mail: [email protected] D. R. Boreham Bruce Power, Integration Department, Tiverton, ON N06 2T0, Canada D. R. Boreham Department of Medical Physics and Applied Radiation Sciences, McMaster University, Hamilton, ON L8S 4K1, Canada K. L. Jones Department of Biochemistry and Molecular Genetics, School of Medicine, University of Colorado, Aurora, CO 80045, USA
North America; 15 of the 22 loci produced scorable PCR products in the round whitefish. Keywords Prosopium Microsatellite PAL_FINDER PCR primers SSR
The mountain whitefish, Prosopium williamsoni, is one of six members of the genus Prosopium, (the round whitefishes) within the Coregoninae of the family Salmonidae (salmon, trout and relatives). The geographic range of P. williamsoni encompasses rivers and lakes from the British Columbia-Yukon border area in northwestern North America south to the Lahontan Basin in Nevada. While primarily found west of the Continental Divide, its range includes areas east of the divide in the Liard, Peace, Athabasca, Saskatchewan, Milk, and upper Missouri rivers (Scott and Crossman 1973). Throughout this area, P. williamsoni is often an abundant member of the fish community with an important role as a forage and game fish (Scott and Crossman 1973) and its status has been ranked as ‘‘vulnerable’’ by NatureServe (2012) in several jurisdictions. Studies of the population genetic structure of this fish, however, have been hampered a lack of suitable genetic resources. Total DNA was extracted from o
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