Development of PCR-based markers and whole-genome selection model for anthracnose resistance in white lupin ( Lupinus al
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PLANT GENETICS • ORIGINAL PAPER
Development of PCR-based markers and whole-genome selection model for anthracnose resistance in white lupin (Lupinus albus L.) Sandra Rychel-Bielska 1,2 Michał Książkiewicz 2
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Nelson Nazzicari 3 & Piotr Plewiński 2
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Wojciech Bielski 2
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Paolo Annicchiarico 3
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Received: 11 July 2020 / Revised: 7 September 2020 / Accepted: 14 September 2020 # The Author(s) 2020
Abstract White lupin (Lupinus albus L.) is a high-protein grain legume crop, grown since ancient Greece and Rome. Despite long domestication history, its cultivation remains limited, partly because of susceptibility to anthracnose. Only some late-flowering, bitter, low-yielding landraces from Ethiopian mountains displayed resistance to this devastating disease. The resistance is controlled by various genes, thereby complicating the breeding efforts. The objective of this study was developing tools for molecular tracking of Ethiopian resistance genes based on genotyping-by-sequencing (GBS) data, envisaging linkage mapping and genomic selection approaches. Twenty GBS markers from two major quantitative trait loci (QTLs), antr04_1/antr05_1 and antr04_2/antr05_2, were converted to PCR-based markers using assigned transcriptome sequences. Newly developed markers improved mapping resolution around both anthracnose resistance loci, providing more precise QTL estimation. PCR-based screening of diversified domesticated and primitive germplasm revealed the high specificity of two markers for the antr04_1/ antr05_1 locus (TP222136 and TP47110) and one for the antr04_2/antr05_2 locus (TP338761), highlighted by simple matching coefficients of 0.96 and 0.89, respectively. Moreover, a genomic selection approach based on GBS data of a recombinant inbred line mapping population was assessed, providing an average predictive ability of 0.56. These tools can be used for preselection of candidate white lupin germplasm for anthracnose resistance assays. Keywords White lupin . Marker-assisted selection . Genomic selection . Anthracnose resistance . Quantitative trait
Introduction
Communicated by: Izabela Pawłowicz Electronic supplementary material The online version of this article (https://doi.org/10.1007/s13353-020-00585-1) contains supplementary material, which is available to authorized users. * Michał Książkiewicz [email protected] 1
Department of Genetics, Plant Breeding and Seed Production, Wroclaw University of Environmental and Life Sciences, Plac Grunwaldzki 24A, 50-363 Wrocław, Poland
2
Institute of Plant Genetics, Polish Academy of Sciences, Strzeszyńska 34, 60-479 Poznań, Poland
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CREA-FLC, Council for Agricultural Research and Economics, Research Centre for Fodder Crops and Dairy Production, Viale Piacenza 29, 26900 Lodi, Italy
Lupins are valuable crops appreciated as a source of protein for food and feed, as well as plants enhancing mobilization of soil phosphorus, improving soil fertility through symbiotic nitrogen fixation, and increasing economic payback for succeeding crops (Lambers et al. 2013). The seed of mo
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