Enhancing of anthracnose disease resistance indicates a potential role of antimicrobial peptide genes in cassava
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ORIGINAL PAPER
Enhancing of anthracnose disease resistance indicates a potential role of antimicrobial peptide genes in cassava Pattaraporn Hormhuan1,2 · Unchera Viboonjun3 · Punchapat Sojikul1 · Jarunya Narangajavana1,2 Received: 25 March 2020 / Accepted: 3 July 2020 © Springer Nature Switzerland AG 2020
Abstract Cassava (Manihot esculenta Crantz.) is an important economic crop in tropical countries. Demands for using cassava in food, feed and biofuel industries have been increasing worldwide. Cassava anthracnose disease, caused by Colletotrichum gloeosporioides f.sp. manihotis (CAD), is considered a major problem in cassava production. To minimize the effects of such disease, this study investigated the response of cassava to attack by CAD and how the plants defend themselves against this threat. Genome-wide identification of antimicrobial peptide genes (AMPs) and their expression in response to fungal infection was performed in the resistant cassava cultivar (Huay Bong 60; HB60) in comparison with the highly susceptible cultivar (Hanatee; HN). A total of 114 gene members of AMP were identified in the cassava genome database. Fifty-six gene members were selected for phylogenetic tree construction and analysis of putative cis-acting elements in their promoter regions. Differential expression profiles of six candidate genes were observed in response to CAD infection of both cassava cultivars. Upregulation of snakins, MeSN1 and MeSN2 was found in HB60, whereas MeHEL, Me-AMP-D2 and MeLTP2 were highly induced in HN. The MeLTP1 gene was not expressed in either cultivar. HB60 showed a reduced severity rating in comparison to HN after CAD infection. The biomembrane permeability test of fungal CAD was strongly affected after treatment with protein extract derived from CAD-infected HB60. Altogether, these findings suggest that snakins have a potential function in the CAD defense response in cassava. These results could be useful for cassava improvement programs to fight fungal pathogen. Keywords Antimicrobial peptides · Cassava · Cassava anthracnose disease · Snakins
Introduction Cassava (Manihot esculenta Crantz) is the starchy tuberous edible plant of the Euphorbiaceae family. It originated in South America and is typically cultivated in tropical and subtropical region. Cassava tuber is a main source of Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10709-020-00097-0) contains supplementary material, which is available to authorized users. * Jarunya Narangajavana [email protected] 1
Department of Biotechnology, Faculty of Science, Mahidol University, Bangkok, Thailand
2
Center of Excellence on Agricultural Biotechnology (AG-BIO/PERDO-CHE), Bangkok, Thailand
3
Department of Plant Science, Faculty of Science, Mahidol University, Bangkok, Thailand
carbohydrates for consumption as human food, animal feed and various industries including biodegradable plastics and biofuel production (Jansson et al. 2009). As a result of the demand for renewable ene
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