Development of Rapid, Sensitive, and Effective Plasmonic Nanosensor for the Detection of Vitamins in Infact Formula and
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Development of Rapid, Sensitive, and Effective Plasmonic Nanosensor for the Detection of Vitamins in Infact Formula and Milk Samples Duygu ÇIMEN and Adil DENIZLI* Department of Chemistry, Hacettepe University, Ankara-06800, Turkey *
Corresponding author: Adil DENIZLI
E-mail: [email protected]
Abstract: The aim of the present study is to develop a surface plasmon resonance sensor for the detection of vitamin B2, vitamin B9, and vitamin B12 in food samples by using the molecular imprinting technique. The vitamin B2, vitamin B9, and vitamin B12 imprinted and the non-imprinted surface plasmon resonance sensor chip surfaces were characterized by using contact angle measurements, atomic force microscopy, ellipsometry, and Fourier transform infrared-attenuated total reflectance. The real-time detection of vitamin B2, vitamin B9, and vitamin B12 was analyzed by using aqueous solutions in the concentration range of 0.01 ng/mL − 10 ng/mL for vitamin B2, 0.1 ng/mL − 8.0 ng/mL for vitamin B9, and 0.01 ng/mL − 1.5 ng/mL for vitamin B12. The limit of detection values was calculated as 1.6×10−4 ng/mL for vitamin B2, 13.5×10−4 ng/mL for vitamin B9, and 2.5×10−4 ng/mL for vitamin B12, respectively. Selectivity experiments were performed by using vitamin B1 and vitamin B6. The reproducibility of surface plasmon resonance sensors was investigated both on the same day and on different days for four times. Validation studies of the prepared surface plasmon resonance (SPR) sensors were performed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Keywords: Vitamin; molecular imprinting; surface plasmon resonance; food samples Citation: Duygu ÇIMEN and Adil DENIZLI, “Development of Rapid, Sensitive, and Effective Plasmonic Nanosensor for the Detection of Vitamins in Infact Formula and Milk Samples,” Photonic Sensors, DOI: 10.1007/s13320-020-0578-1.
1. Introduction Vitamins are organic compounds that are not produced by body cells. Therefore, vitamins need to be taken from nutrients and supplements daily. They play an important role in carbohydrate, fat and protein metabolism, healthy development of body, immunity against infections, and digestive functions [1−3]. Especially, vitamin B (such as vitamin B2, vitamin B9, and vitamin B12) is very important for human health [4, 5]. The demand of the body for vitamin B2 increases during the growth
and pregnancy in children and women. The deficiency of vitamin B2 can be seen in the visual impairment, disorders of the nervous system, skin wounds, and various areas of the body such as inflammation of the skin. Vitamin B9 deficiency is often seen in elderly, pregnant women, and premature infants. The use of vitamin B9 reduces the risk of disease related to the brain and spinal cord systems during pregnancy in women [6−9]. Genetic structure, division, and features of the cells are used for the development of the central nervous system of the baby in the early stages of pregnancy.
Received: 16 July 2019 / Revised: 19 December 2019 © The Author(s) 2020. This article is published
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