The Development of a Photothermal Immunochromatographic Lateral Flow Strip for Rapid and Sensitive Detection of Bispheno
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The Development of a Photothermal Immunochromatographic Lateral Flow Strip for Rapid and Sensitive Detection of Bisphenol A in Food Samples Yang Lu 1
&
Qian Wang 1 & Chuan Zhang 1 & Shijie Li 1 & Shilun Feng 2 & Shuo Wang 3
Received: 8 April 2020 / Accepted: 25 August 2020 # Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract A novel quantitative photothermal-sensing immunochromatographic assay (PT-ICA) with high sensitivity was developed for the detection of bisphenol A (BPA). Gold acid chloride trihydrate (HAuCl4·3H2O) was first reduced by citric acid trisodium salt (C6H5Na3O7) to form Au nanoparticles and adsorbed on the surface of black phosphorus nanosheets to form a black phosphorusAu (BP-Au) complex. The antibody against BPA was then coupled to the BP-Au complex to form the black phosphorus-Auantibody (BP-Au-Ab) photothermal probe for capturing BPA in samples. During the detection, the precoated antigen BPAbovine serum albumin (BPA-BSA) on the T line and free BPA molecules in the sample would competitively bind to the limited BP-Au-Ab photothermal probes. After that, the exothermic process under an 808-nm laser was recorded using an infrared thermal imager, which represented the concentration of BPA in the samples. The PT-ICA demonstrated high sensitivity with a limit detection of 0.24 ng/mL, which was 6.25 times lower than that of the visual-ICA. In addition, the assays were highly specific, only minor cross-sensitivity occurred between BPA and 4-cumylphenol. The optimized assay was applied in the detection of BPA in different food samples with recoveries ranging from 75.91 to 119.59%. Finally, the PT-ICA was verified using a commercial enzyme-linked immunosorbent assay (ELISA) kit. The results of the developed assay showed good agreement with that of the ELISA kit. The optimized PT-ICA required a shorter detection time (approximately 30 min) than the ELISA kit (approximately 2 h), which has potential applications in the detection field. Keywords Photothermal-sensing immunochromatographic assay . Blackphosphorusnanosheets . Aunanoparticles . BisphenolA
Introduction Bisphenol A (BPA) is an endocrine-disrupting chemical that was first used to promote the growth of poultry owing to its function of estrogenic or androgenic activity (Lombó et al. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s12161-020-01841-0) contains supplementary material, which is available to authorized users. * Yang Lu [email protected] * Shuo Wang [email protected] 1
State Key Laboratory for Food Nutrition and Safety, College of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China
2
School of Electrical and Electronic Engineering, Nanyang Technological University, Singapore 639798, Singapore
3
Medical College, Nankai University, Tianjin 300500, China
2019). It is also one of the most widely used chemicals in industry. It is the source of daily consumer products, including polycarbonate plastics, epox
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