Development of sixteen novel polymorphic microsatellite markers in Rhinogobio ventralis (Teleostei, Cyprinidae, Gobionin
- PDF / 172,413 Bytes
- 3 Pages / 595.276 x 790.866 pts Page_size
- 60 Downloads / 172 Views
MICROSATELLITE LETTERS
Development of sixteen novel polymorphic microsatellite markers in Rhinogobio ventralis (Teleostei, Cyprinidae, Gobioninae) Ke Shao • Shuxiang Yan • Meihua Xiong Weitao Li • Lei Pan • Nian Xu
•
Received: 8 September 2014 / Accepted: 19 September 2014 / Published online: 28 September 2014 Springer Science+Business Media Dordrecht 2014
Abstract In the present study, we developed 16 polymorphic microsatellite loci in Rhinogobio ventralis, an endemic cyprinid fish in the upper reaches of the Yangtze River. The polymorphism of each locus was assessed in 32 individuals collected from Jiangjin section of the Yangtze River. A total of 126 alleles were detected and the number of alleles per locus ranged from 2 to 16. The observed and expected heterozygosities ranged from 0.161 to 0.968 and from 0.154 to 0.928, respectively. Two loci significantly deviated from Hardy–Weinberg equilibrium. No significant linkage disequilibrium was found between pairs of loci. These polymorphic microsatellites will be useful tools in the study of genetic diversity and population structure of R. ventralis. Keywords Rhinogobio ventralis Yangtze River Microsatellite loci Genetic diversity Rhinogobio ventralis is an endemic cyprinid fish in China, which has great economic importance. It used to be widely distributed in the upper reaches of the Yangtze River and its main branches. However, in recent years, due to overfishing and cascade hydropower development, the natural resource of R. ventralis has significantly declined
Electronic supplementary material The online version of this article (doi:10.1007/s12686-014-0341-2) contains supplementary material, which is available to authorized users. K. Shao S. Yan M. Xiong W. Li L. Pan N. Xu (&) Key Laboratory of Ecological Impacts of Hydraulic-Projects and Restoration of Aquatic Ecosystem of Ministry of Water Resources, Institute of Hydroecology, Ministry of Water Resources and Chinese Academy of Sciences, 578th Xiongchu Avenue, Wuhan 430079, Hubei, People’s Republic of China e-mail: [email protected]; [email protected]
and even faced the risk of extinction in its historical range. To better address the conservation status of this species in the Yangtze River, it’s essential to gain a better understanding of its population genetic structure. With a rising number of microsatellites, the power to assess the population genetic diversity can be increased. To date, only twelve polymorphic microsatellites for R. ventralis have been published (Xu et al. 2010), of which two pairs of loci were found with linkage disequilibrium and three loci showed significant departures from Hardy–Weinberg equilibrium when tested on a population of 40 individuals. Therefore, more loci are needed to estimate genetic structure. Herein, we report 16 novel polymorphic microsatellites developed for R. ventralis, which will facilitate conservation genetics studies of this species. A genomic library enriched for the repeat motif (GATA)n was constructed, using the fast isolation by AFLP
Data Loading...
