Development of eight polymorphic microsatellite markers in the Black and Rufous sengi, Rhynchocyon petersi
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MICROSATELLITE LETTERS
Development of eight polymorphic microsatellite markers in the Black and Rufous sengi, Rhynchocyon petersi Christopher A. Sabuni • Natalie Van Houtte • Samwel L. S. Maganga • Rhodes H. Makundi Herwig Leirs • Joe¨lle Gou¨y de Bellocq
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Received: 26 August 2014 / Accepted: 28 August 2014 / Published online: 14 September 2014 Ó Springer Science+Business Media Dordrecht 2014
Abstract The Black and Rufous sengi, Ryhnchocyon petersi, is endemic to a limited range in East Africa. We report the development of eight polymorphic microsatellites using next generation sequencing technology. Eighteen individuals from Zaraninge forest (Saadani National Park, Tanzania) were genotyped. The number of alleles per locus ranged from 2 to 6, while the observed and expected heterozygosities varied from 0.17 to 0.82 and from 0.25 to 0.81, respectively. No locus deviated from Hardy–Weinberg equilibrium. These microsatellite markers will be useful tools to study the effect of habitat fragmentation on the population genetic structure of R. petersi. Keywords Rhynchocyon petersi Vulnerable afrotherian Microsatellites 454 Sequencing
Electronic supplementary material The online version of this article (doi:10.1007/s12686-014-0330-5) contains supplementary material, which is available to authorized users. C. A. Sabuni R. H. Makundi Pest Management Centre, Sokoine University of Agriculture, Box 3110, Morogoro, Tanzania N. Van Houtte H. Leirs J. Gou¨y de Bellocq Evolutionary Ecology Group, University of Antwerp, Groenenborgerlaan 171, 2020 Antwerp, Belgium S. L. S. Maganga Department of Wildlife Management, Sokoine University of Agriculture, Box 3073, Morogoro, Tanzania J. Gou¨y de Bellocq (&) Research Facility Studenec, Institute of Vertebrate Biology, Academy of Sciences of the Czech Republic, Kveˇtna´ 8, Brno, Czech Republic e-mail: [email protected]
Rhynchocyon petersi Bocage, 1880, the Black and Rufous sengi (elephant-shrew) is one of the four known giant sengis from the subfamily Rhynchocyoninae which belong to the supercohort Afrotheria grouping together aardvark, tenrecs, golden-moles, elephants, hyraxes and sea-cows. This species is endemic to East Africa, distributed in the Eastern Arc Mountains and costal forests of Tanzania and Kenya. These habitats show a high level of biodiversity and endemism but are threatened by fragmentation and degradation due to agricultural expansion, timber for various uses and charcoal production (Rathbun and Butynski 2008). Rhynchocyon petersi is categorized as vulnerable due to the loss of its habitats (IUCN 2014). Little is known about this species because no detailed field studies have ever been conducted (Rathbun and Butynski 2008). Our aim was to develop microsatellite loci for future genetic studies. DNA was extracted using Nucleopsin kit (Macherey Nagel) from a piece of ear. The library construction and microsatellite optimisation were outsourced to Genoscreen (Lille, France). The high-throughput method used by Genoscreen is based on coupling multiplex micros
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