Diagnostic and Prognostic Importance of AgNOR Pleomorphism in Cervical Carcinoma
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ORIGINAL ARTICLE
Diagnostic and Prognostic Importance of AgNOR Pleomorphism in Cervical Carcinoma Gangotree Mohanty1
•
Ashok Kumar Padhy2
Received: 7 June 2020 / Revised: 26 July 2020 / Accepted: 30 July 2020 Ó Association of Gynecologic Oncologists of India 2020
Abstract Introduction Nucleolar organizer region-associated proteins are argyrophilic and visualized by silver staining called AgNOR. It can be used as a diagnostic and prognostic marker for malignant cells which exhibit a greater AgNOR protein. Aim The present study aimed to evaluate the correlation of AgNOR counts with severity and prognosis of cervical lesion. Materials and Methods Silver nitrate staining was performed in a total of 50 cervical smears collected from the women coming to the outpatient department of the hospital diagnosed as cytological normal, inflammatory, low grade squamous intraepithelial lesion, high grade squamous intraepitelial lesion and squamous cell carcinoma (SCC). Total cell counts were determined for single and pleomorphic dots. Results Both single and pleomorphic dots increase with an increase in grades of lesions from cytological normal to SCC. But mean cell counts of pleomorphic dots increase with cytological abnormality. Correlation of disease severity with a cell count of pleomorphic dots (r = 0.96, p B 0.001) and single dots (r = 0.98, p B 0.001) is positive and significant. Conclusion A significant increase in pleomorphic dot in patients indicated the prognostic importance of pleomorphic counts during the increase in cytological abnormalities of cervical lesions. Keywords AgNOR LSIL HSIL Pleomorphic dots
Introduction Cervical cancer is the second most common cancer in women of reproductive age group after breast cancer. Early and accurate screening of cytological abnormalities at minimum cost is expected to reduce the incidence of cervical cancer and deaths in developing countries. Nucleolar organizer regions (NORs) are chromosomal segments, which contain ribosomal genes. NORs also contain a set of acidic, nonhistone proteins that bind silver ions and are selectively visualized by silver staining methods in routinely processed cyto-histological samples [1]. Currently, various studies are being conducted to explore the possibility & Gangotree Mohanty [email protected] Ashok Kumar Padhy [email protected] 1
Ravenshaw University, Cuttack, Odisha 753003, India
2
Department of Gynaecology and Obstratics, Acarya Harihar Regional Cancer Centre, Cuttack, Odisha 753003, India
of finding out the tumor marker potential of AgNOR dots [2]. This has been necessitated because the technique is very easy, inexpensive and less time-consuming and provides accurate information on neoplastic development of cervical lesions. It is used for identification of precancerous lesion by cytological screening of cervical cells. In the present study, AgNOR dots, both pleomorphic and single, have been counted in cervical smears under different pathological conditions of the uterine cervix and the data have been analyzed
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